乌贼DNA条形码的筛选与验证

Selection and verification of DNA barcoding for cuttlefish

中文关键词:  DNA条形码  引物组  优化  乌贼鉴定

英文关键词:DNA barcoding  primer sets  optimization  cuttlefish identification

中文摘要:

      目的 筛选适于鉴定乌贼的DNA条形码, 建立鉴定舟山常见乌贼种类的DNA条形码技术体系。方法 用聚合酶链反应(polymerase chain reaction, PCR)对现有5组DNA条形码引物组合进行了筛选, 设计一对新的DNA条形码引物以供筛选备用。结果 现有引物与部分乌贼的DNA模板存在错配而缺乏通用性, 在部分乌贼DNA的扩增受阻。本实验设计的12S rRNA基因引物可以特异性扩增乌贼的DNA, 提高了乌贼鉴定的准确度。结论 本研究设计的12S rRNA基因引物可以作为现有乌贼DNA条形码鉴定的补充。

英文摘要:

      Objective To find the most suitable DNA barcoding primers and develop the DNA barcoding technology system for identification of Zhoushan cuttlefish species. Methods Five existing DNA barcoding primer sets were chosen and optimized by polymerase chain reaction (PCR), and another new primer pair was designed for further selection in this study. Results The existed 5 DNA barcoding primer sets had mismatched base with part of cuttlefish DNA samples, which resulted in PCR amplification was blocked in some cuttlefish species. The new designed pair of primer pair could amplify cuttlefish 12S rRNA region specifically, which improved the efficiency and accuracy of species identification. Conclusion The 12S rRNA gene primer designed in this experiment can be used as a supplement to the identification of existing squid DNA barcodes.