超高效液相色谱–质谱联用法定量测定奶茶中的 3种毒品成分

Quantitative determination of 3 related narcotic substances in milk tea by ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  奶茶  毒品  超高效液相色谱法  三重四极杆质谱法

英文关键词:milk tea  narcotic substances  ultra performance liquid chromatography  triple quadrupole mass spectrometry

中文摘要:

      目的 建立超高效液相色谱质谱联用法测定奶茶中苯丙胺、甲基苯丙胺和氯胺酮的分析方法。 方法 样品经甲醇超声提取处理, 利用超高效液相色谱质谱联用法, 4.0 min内完成样品的上机分析。结果 方法在0.1~50.0 ng/mL范围内, 线性良好, 相关系数r大于0.999。仪器检出限在0.008~0.027 ng/mL之间, 定量限在0.028~0.089 ng/mL之间。三水平加标回收率在80.7%~112.0%之间。高浓度样品分析后, 3种毒品无明显仪器残留。使用本方法分析市售奶茶样品, 未检出3种毒品。结论 本方法灵敏、快速、线性范围宽、选择性好、前处理简单, 适用于奶茶中3种毒品的准确定量检测。

英文摘要:

      Objective To establish a method for determining amphetamine, methamphetamine and ketamine in milk tea by ultra performance liquid chromatography-tandem mass spectrometry. Methods The sample was ultrasonically extracted by methanol, utilizing ultra performance liquid chromatography-mass spectrometry, the sample analysis of the sample was completed within 4.0 min. Results In the range of 0.1?50.0 ng/mL, the linearity was good, and the correlation coefficient r was greater than 0.999. The limit of detection of the instrument was in 0.008?0.027 ng/mL, and the limit of quantification was in 0.028?0.089 ng/mL. The recoveries rate of the 3 levels was in 80.7%?112.0%. After analyzing the high-concentration samples, there were no obvious instrument residues in the 3 drugs. The method was used to analyze commercially available milk tea samples, and the 3 drugs were not detected. Conclusion This method is sensitive, rapid, wide in linear range, good in selectivity, simple in pretreatment, and suitable for accurate quantitative detection of 3 drugs in milk tea.