免疫磁珠分离技术在食源性致病菌检测中的应用

Application of immunomagnetic beads separation techniques in detection of foodborne pathogenic bacteria

中文关键词:  免疫磁珠  免疫磁珠分离技术  食源性致病菌

英文关键词:immunomagnetic beads  immunomagnetic beads separation techniques  foodborne pathogens

中文摘要:

      免疫磁珠(immunomagnetic beads, IMB)是一种均匀、具有超顺磁性及保护性壳的球形小粒子, 由载体微球和免疫配基结合而成。免疫磁珠分离技术(immunomagnetic beads separation techniques, IMBS)则是利用免疫磁珠上包被的特异性抗体与抗原发生亲和反应, 从复杂的样品中分离到目标抗原。再利用磁珠的磁响应性, 实现对目标抗原的富集。在食源性致病菌检测方面, 该技术通过与其他检测手段相结合而得到广泛应用, 具有灵敏度高、检测时间短、操作简单等优势。本文综述了免疫磁珠的结构特点、免疫磁珠分离技术的原理, 着重阐述了该技术在食源性致病菌检测方面的应用进展, 以期为免疫磁珠分离技术的广泛应用提供参考。

英文摘要:

      Immunomagnetic beads (IMB) is a kind of uniform, has superparamagnetism and protective shell of small spherical particles, by the carrier on the combination of microspheres and immune ligands. The immunomagnetic bead separation techniques (IMBS) use the specific antibody coated on the immunomagnetic beads to affinity with the antigen to separate the target antigen from the complex sample. In the detection of foodborne pathogens, this technology has been widely used by combining with other detection methods, and has the advantages of high sensitivity, short detection time and simple operation. This paper reviewed the structural characteristics of immunomagnetic beads and the principles of immunomagnetic beads separation techniques, emphasized the application progress of this technology in the detection of foodborne pathogenic bacteria, so as to provide references for the wide application of immunomagnetic separation techniques.