超高效液相色谱法测定清咽糖中6种绿原酸类化合物的含量

Determination of 6 kinds of chlorogenic acid compounds content in Qingyan sugar by ultra performance liquid chromatography

中文关键词:  清咽糖  新绿原酸  绿原酸  隐绿原酸  3,4-O-二咖啡酰基奎宁酸  3,5-O-二咖啡酰基奎宁酸  4,5-O-二咖啡酰基奎宁酸  超高效液相色谱法

英文关键词:Qingyan sugar  neochlorogenic acid  chlorogenic acid  cryptochlorogenic acid  3,4-O-dicaffeoylquinic acid  3,5-O-dicaffeoylquinic acid  4,5-O-dicaffeoylquinic acid  ultra performance liquid chromatography

中文摘要:

      目的 建立超高效液相色谱法同时测定清咽糖中新绿原酸、绿原酸、隐绿原酸、3,4-O-二咖啡酰基奎宁酸、3,5-O-二咖啡酰基奎宁酸和4,5-O-二咖啡酰基奎宁酸6种绿原酸类化合物的含量。方法 采用Agilent Eclipse Plus C18 RRHD色谱柱(2.1 mm×150 mm, 1.8 μm), 以乙腈、0.4%磷酸水溶液为流动相, 梯度洗脱, 流速0.8 mL/min, 检测波长327 nm, 柱温35 ℃。结果 6种成分在相应的线性范围内呈良好的线性关系。精密度、重复性、稳定性实验中6种成分峰面积的相对标准偏差均小于3.0%, 平均加样回收率为93.77%~104.37%, 相对标准偏差为0.28%~1.86%。结论 该方法具有良好的专属性和重现性, 加样回收率实验符合要求, 结果准确、稳定, 适用于同时测定清咽糖中新绿原酸、绿原酸、隐绿原酸、3,4-O-二咖啡酰基奎宁酸、3,5-O-二咖啡酰基奎宁酸和4,5-O-二咖啡酰基奎宁酸的含量。

英文摘要:

      Objective To establish a method for simultaneous determination the content of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid and 4,5-O- dicaffeoylquinic acid in Qingyan sugar by ultra performance liquid chromatography. Methods The samples were separated with a Agilent Eclipse Plus C18 RRHD column (2.1 mm×150 mm, 1.8 μm) at the flow rate of 0.8 mL/min by gradient elution, using acetonitrile and 0.4% phosphate solution as the mobile phase, the detection wavelength was 327 nm, and the column temperature was 35 ℃. Results The 6 components showed a good linear relationship in the corresponding linear range. The relative standard deviations of the peak areas of the 6 components in the precision, repeatability and stability experiments were all less than 3.0%, the average recoveries were 93.77%?104.37%, and the relative standard deviations were 0.84%?1.64%. Conclusion This method has good specificity and reproducibility, the experiment of recovery rate meets the requirement, and the results are accurate and stable. It is suitable for the simultaneous determination of the content of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 3,4-O-dicoffee acyl quininic acid, 3,5-O-dicoffee acyl quininic acid and 4,5-O-dicoffee acyl quininic acid in Qingyan sugar.