响应面法优化白芷水溶性多糖提取工艺的研究

Optimization of extraction process of water soluble polysaccharide from Angelica dahurica by response surface methodology

中文关键词:  白芷多糖  提取工艺  优化  响应面分析法

英文关键词:Angelica dahurica polysaccharide  extraction process  optimization  response surface methodology

中文摘要:

      目的 采用响应面法优化白芷水溶性多糖的提取工艺。方法 用苯酚-硫酸比色法测定白芷水溶性多糖在波长490 nm处的吸光度, 计算多糖提取率并以此为实验指标, 在单因素实验的基础之上, 选取提取温度、提取时间、液料比为考察因素, 利用Box-Bebnken方法进行三因素三水平实验设计。结果 单因素最佳试验条件为液料比100:1(mL:g), 提取时间90 min, 提取温度70 ℃; 响应面法优选出白芷水溶性多糖最佳提取工艺参数为液料比102:1(mL:g), 提取时间90 min, 提取温度68 ℃, 在该优化条件下多糖实际提取率7.35%±0.005%。结论 本研究优化的白芷水溶性多糖的提取工艺方便、稳定。

英文摘要:

      Objective To optimize the extraction process of water-soluble polysaccharides from Angelica dahurica Alba by response surface methodology. Methods Phenol-sulfuric acid colorimetric method was used to determine the absorbance of water-soluble polysaccharides from Angelica dahurica at 490 nm. The extraction rate of polysaccharides was calculated as an experimental index. On the basis of single factor experiment, the extraction temperature, extraction time and liquid-material ratio were selected as the factors to investigate. Box-Bebnken method was used to design three-factor and three-level experiment. Results The optimum conditions for single factor extraction were as follows: the ratio of liquid to material was 100:1 (mL:g), the extraction time was 90 min, and the extraction temperature was 70 ℃. Response surface methodology was used to optimize the extraction parameters of Angelica dahurica water-soluble polysaccharides: the ratio of liquid to material was 102:1 (mL:g), the extraction time was 90 min, and the extraction temperature was 68 ℃. Under the optimum conditions, the actual extraction rate of polysaccharides was 7.35%±0.005%. Conclusion The optimized extraction process of water- soluble polysaccharides from Radix Paeoniae Alba is convenient and stable.