甲醇萃取法在食用油中酚类抗氧化剂检测中的应用研究

Study on the application of methanol extraction in the determination of phenolic antioxidants in edible oil

中文关键词:  食用油  抗氧化剂  甲醇萃取法

英文关键词:edible oil  antioxidant  methanol extraction

中文摘要:

      目的 对比国标方法与甲醇萃取法萃取食用油中的酚类抗氧化剂。方法 分别用乙酸乙酯:环己烷(1:1, V:V)萃取凝胶渗透色谱仪(gel permeation chromatograph, GPC)进行样品净化气相色谱检测和甲醇萃取冷冻过滤气相色谱检测。结果 甲醇萃取法在检测叔丁基羟基茴香醚(butyl hydroxyl acids, BHA)、2, 6-二叔丁基-4-甲基苯酚(butylated hydroxytoluene, BHT)、 叔丁基对苯二酚(tertiary butylhydroquinone, TBHQ) 3种抗氧化剂在各自的线性范围内, 线性关系良好, 相关系数r大于0.999, 相对标准偏差(relative standard deviation RSD)为0.91%~1.75%, 回收率为89%~98%。结论 该方法简单、快速、准确、实验成本低, 适用于食用油中酚类抗氧化剂的测定。

英文摘要:

      Objective To compare the extraction of phenolic antioxidants in edible oil by the national standard method and methanol extraction method. Methods Extracted with ethyl acetate: cyclohexane (1:1, V: V), respectively, using a gel permeation chromatograph (GPC) for sample purification gas chromatography and methanol extraction freeze filtration gas chromatography. Results Methanol extraction method for the detection of butyl hydroxyl acids (BHA), 2, 6-di-tert-butyl-4-methylphenol (BHT), tert-butyl hydroquinone (tertiary butylhydroquinone), TBHQ) The three antioxidants have good linearity in their respective linear ranges, the correlation coefficient was greater than 0.999, the relative standard deviations (RSDs) were 0.91% to 1.75%, and the recovery were 89% to 98%. Conclusion This method is simple, rapid, accurate and low in experimental cost, which is suitable for the determination of phenolic antioxidants in edible oils.