高效液相色谱-电感耦合等离子体质谱法测定婴幼儿谷类辅助食品中无机砷及其他砷形态

Determination of inorganic arsenic and other arsenic species in infant cereals by high performance liquid chromatography-inductively coupled plasma mass spectrometry

中文关键词:  无机砷  砷形态  婴幼儿谷类辅助食品  高效液相色谱法  电感耦合等离子体质谱法

英文关键词:inorganic arsenic  arsenic species  infant cereals  high performance liquid chromatography  inductively coupled plasma mass spectrometry

中文摘要:

      目的 建立高效液相色谱-电感耦合等离子体质谱法测定婴幼儿谷类辅助食品中无机砷及其他砷形态的分析方法。方法 婴幼儿谷类辅助食品经0.15 mol/L硝酸溶液提取后, 采用阴离子分析柱, 以不同浓度的碳酸铵溶液作为流动相进行梯度洗脱分离, 用高效液相色谱-电感耦合等离子体质谱进行定性和定量分析。 结果 建立的5种砷形态分析方法在0~100 μg/L范围内具有良好的线性关系, 相关系数r>0.999, 加标回收率在89.0%~110.0%, 相对标准偏差(relative standard deviation, RSD)<5%。婴幼儿谷类辅助食品中砷形态以无机砷为主, 在调查的样品中无机砷的含量均远低于国家食品标准的限量。结论 该方法简便、准确, 适用于婴幼儿谷类辅助食品中无机砷及其他砷形态的测定及食品安全的实际监测。

英文摘要:

      Objective To establish a method for investigation of inorganic arsenic and other arsenic species in cereal-based complementary food for infants and young children (infant cereals) by high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). Methods The arsenic species in infant cereals were extracted by 0.15 mol/L nitric acid solution, separated by gradient elution with different concentrations of ammonium carbonate as the mobile phase on anion analysis column, and analyzed both quantitatively and qualitatively by HPLC-ICP-MS. Results The established method for 5 arsenic species analysis had good linearity in the concentration range of 0?100 μg/L, correlation coefficient of all the above 0.999 with the recovery rates of was 89.0%?110.0%, and relative standard deviation of less than 5%. The main forms of arsenic species in the infant cereal samples detected were inorganic arsenic. The content of inorganic arsenic in the samples investigated was all far lower than the national food standard limit. Conclusion This method is simple and accurate for the detection of inorganic arsenic and other arsenic species in infant cereals and could be applied in monitoring practice.