超高效液相色谱-串联质谱法检测动物血液中6种β-受体激动剂类药物残留

Determination of 6 kinds of β-agonists residues in animal blood by ultra performance liquid chromatography tandem mass spectrometry

中文关键词:  β-受体激动剂  动物血液  超高效液相色谱-串联质谱法

英文关键词:β-agonists  animal blood  ultra performance liquid chromatography tandem mass spectrometry

中文摘要:

      目的 建立超高效液相色谱-串联质谱法检测动物血液样品中6种β-受体激动剂类药物残留的分析方法。方法 样品经乙腈提取, 经Waters Acquity UPLC BEH C18色谱柱分离, 以乙腈?0.1%甲酸溶液为流动相进行梯度洗脱, 电喷雾正离子(electrospray ionization, ESI+)模式电离, 多反应监测(multiple reaction monitoring, MRM)扫描模式检测。结果 6种化合物在0.2~50 ng/mL范围内均呈现良好的线性关系, 相关系数(r)均大于0.990。检出限为0.04~0.11 μg/kg, 猪血液样品中3个浓度添加水平平均回收率为86.2%~99.2%, 相对标准偏差为3.2%~8.4%。结论 本方法操作简便, 回收率高, 适用于动物血液样品中6种β-受体激动剂类药物同时定性、定量分析。

英文摘要:

      Objective To establish a method for the determination of 6 kinds of β-agonists residues in animal blood by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods Samples were extracted with acetonitrile solution, and separated by Waters Acquity UPLC system with a BEH C18 column. The mobile phases were acetonitrile and 0.1% formic acid aqueous solution in gradient elution. The electrospray was operated in the positive ionization mode, and the compounds were identified by multiple reaction monitoring (MRM) mode. Results A total of 6 kinds of compounds had good linear relationships in the range of 0.2?50 ng/mL, and the correlation coefficients were larger than 0.990. The limits of detection were 0.04?0.11 μg/kg. The recoveries at 3 spiked levels were 86.2%?99.2%, with the relative standard deviations of 3.2%?8.4%. Conclusion This method is simple to operate and has high recovery rate, which is suitable for simultaneous qualitative and quantitative analysis of 6 kinds of β-agonists in animal blood samples.