雉鸡益生菌MCm2的分离及鉴定

Isolation and identification of probiotics MCm2 from Phasianus colchicus

中文关键词:  乳酸菌  酸耐受性  胆盐耐受性  16S rDNA

英文关键词:lactobacillus  acid tolerance  bile tolerance  16S rRNA

中文摘要:

      目的 从野雉鸡肠道得到的菌株MCm2并测试其益生活性。方法 通过形态、生理生化和16S rDNA序列分析鉴定菌株, 通过体外试验测试其益生性。结果 菌株在pH 2.0培养2 h后仍能达到2.6×104 CFU/mL; 6 h内, 加胆盐(0.3%~0.4%)对菌株生长量影响很小。经16S rDNA系统发育和recA基因特定片段分析, 菌株MCm2为植物乳杆菌。菌株MCm2培养36 h后, 乳酸含量为24.7 μg/mL, 而pH值下降至3.7。结论 植物乳杆菌MCm2具有益生作用添加到食品中的潜能。

英文摘要:

      Objective To isolated strain MCm2 from Phasianus colchicums and test its probiotics ability. Methods The strains were identified by morphological, physiological, biochemical and 16S rDNA sequence analysis, and its probiotics were tested by in vitro tests. Results The strain could still reach 2.6×104 CFU/mL after 2 h incubation at pH 2.0. Within 6 h, bile salt (0.3%?0.4%) had little effect on the growth of the strain. The strain MCm2 was identified as Lactobacillus plantarum by 16S rDNA phylogeny and specific fragment analysis of recA gene. After 36 h incubation of strain MCm2, the lactic acid content was 24.7 μg/mL, and the pH decrease to 3.7. Conclusion Lactobacillus plantarum MCm2 has the potential to be added to foods as probiotics.