菌株传代及葡萄糖浓度对回复突变实验的影响

Effect of strain passage and glucose concentration on bacterial reverse mutation test

中文关键词:  传代  葡萄糖浓度  回复突变

英文关键词:subculture  glucose concentration  Ames

中文摘要:

      目的 探讨菌株传代培养、不同葡萄糖浓度对回复突变实验的影响。方法 实验菌株选择鼠伤寒沙门氏菌TA型菌株, 分别为TA97、TA98、TA100和TA102菌株, 分别使用原代、传代一次、传代二次菌株进行自发回变实验检测。选取TA97菌株分别在标准底层培养基和低糖顶层培养基进行突变的检测。 结果 4种菌原代组、F1组、F2组的自发回变菌落数差异有统计学意义(P<0.05), 且TA97和TA98的F1组、F2组, TA100的F2组不符合国标GB 15193.4-2014《食品安全国家标准 细菌回复突变试验》对回变菌落数的要求; 标准底层培养基与低糖培养基TA97回复突变数有统计学差异(P<0.05), 但均达到国家标准要求。 结论 菌株的传代及葡萄糖浓度对回复突变实验均有影响。

英文摘要:

      Objective To investigate the effect of the culture of strain passage and different glucose concentrations on the bacterial reverse reversion mutation test. Methods The Salmonella typhimurium TA were selected for strains, which were TA97, TA98, TA100 and TA102 strains, respectively, using the original, sub-generation, and second-generation strains for spontaneous reversal experiments. TA97 strains were selected for mutation detection in standard bottom medium and low-sugar top medium respectively. Results There were significant differences in the number of spontaneously reverting colonies between the 4 strains of origin group, F1 group and F2 group (P<0.05). F1 group, F2 group of TA97 and TA98, F2 group of TA100did not meet the national standard GB 15193.4-2014 National food safety standard-Bacterial reverse mutation assay requirements for the number of reverting colonies. There was a statistically significant difference in the number of reverse mutation between the normal glucose concentration bottom medium and the low concentration medium (P<0.05), but all met the national standards. Conclusion Subculture of the strain and glucose concentration have an effect on the mutation test.