液相色谱-质谱联用法检测特殊医学配方食品中泛酸的含量

Determination of pantothenic acid in special medical formula food by high performance liquid chromatography

中文关键词:  特殊医学配方  液质联用  泛酸  酸碱沉淀

英文关键词:special medical formula  high performance liquid chromatography-mass spectrometry  pantothenic acid  acid-base precipitation

中文摘要:

      目的 建立一种快速准确高效的方法检测特殊医学配方食品中泛酸的含量。 方法 试样中的泛酸, 用酸碱沉淀杂质, 水超声提取, 经Agilent Eclipse XDB-phenyl柱分离, 以甲醇和0.1%甲酸溶液为流动相, 梯度洗脱, 流速为0.4 mL/min, 柱温40 ℃, 采用液相色谱-质谱法检测。结果 泛酸在0.4~7.6 ?g/mL的浓度范围内, 线性关系良好, 相关系数r均大于0.99。平均回收率在90.79%~106.93%之间, 相对标准偏差(relative standard deviation, RSD)为6.3%, 且在6 h内含量保持稳定, 精密度的相对标准偏差(RSD)为1.5%。结论 液质联用法能准确快速高效检测特殊医学配方食品中的泛酸含量, 能对特殊医学用途配方食品中泛酸的含量起到质量控制的目的。

英文摘要:

      Objective To establish a rapid, accurate and efficient method for the detection of pantothenic acid in special medical formula foods for health products. Methods Pantothenic acid in the sample was precipitated by acid and alkali and ultrasonically extracted by water, then separated by Agilent Eclipse XDB-phenyl column at 40 ℃ with methanol and 0.1% formic acid solution as mobile phase at a flow rate of 0.4 mL/min, and detected by liquid chromatography-mass spectrometry. Results The linear relationship of pantothenic acid in the concentration range of 0.4?7.6 ?g/mL was good, and the correlation coefficient r was more than 0.99. The average recovery was between 90.79% and 106.93%, the relative standard deviation (RSD) was 6.3%. The content remained stable within 6 hours, and the relative standard deviation (RSD) of precision was 1.5%. Conclusion Liquid-mass spectrometry can accurately and quickly detect the pantothenic acid content in special medical formula foods, and can control the content of pantothenic acid in formulas for special medical products.