高效液相色谱-蒸发光散射法检测无糖饮料中7种糖类物质的含量

Determination of 7 kinds of sugar substances in sugar-free beverage by high performance liquid chromatography-evaporative light scattering dection

中文关键词:  高效液相色谱-蒸发光散射法  饮料  糖类

英文关键词:high performance liquid chromatography-evaporative light scattering  beverage  sugar

中文摘要:

      目的 建立高效液相色谱-蒸发光散射法检测无糖饮料中7种糖类物质的含量。方法 样品以水为提取剂超声提取15 min, 过聚四氟乙烯滤膜后, 滤液直接上机进行检测。采用prevail carbohydrate ES 5U色谱柱(74.6 mm×250 mm, 5 μm), 以0.04%氨水-乙腈为流动相, 梯度洗脱, 柱温为30 ℃; 流速为1.5 L/min, 外标法定量分析。结果 三氯蔗糖等7种糖类物质在相应的浓度范围内, 线性相关良好, 相关系数均大于0.995, 平均回收率为71.8%~114.4%, 相对标准偏差为0.6%~4.9%。结论 本方法操作简单, 灵敏, 可用于饮料中7种常见糖类物质的检测。

英文摘要:

      Objective To establish a method for the determination of 7 kinds of sugars in sugar-free beverages by high performance liquid chromatography-evaporation spectroscopy. Methods The samples were extracted by ultrasound with water as the extracting agent for 15 min. After passing through the polytetrafluoroethylene (PTFE) filter membrane, the filtrate was directly tested on the machine. The samples were separated with a prevail carbohydrate ES 5U column (74.6 mm ×250 mm, 5 μm) at the flow rate of 1.5 L/min by gradient elution using 0.04% ammonia-acetonitrile as the mobile phase. The temperature of the column oven was maintained at 30 ℃, and the contents were quantitatively analyzed by external standard method. Results Totally 7 kinds of saccharides such as sucralose had good linear correlation in the corresponding concentration range, the correlation coefficient was greater than 0.995, the average recoveries were 71.8%?114.4%, and the relative standard deviations were 0.6%?4.9%. Conclusion The method is simple, sensitive, and can be used for the detection of 7 kinds of common sugar substances in beverages.