一起疑似产气荚膜梭菌食物中毒事件的病原学分析

Etiological analysis of a suspected food poisoning incidents of Clostridium perfringens

中文关键词:  产气荚膜梭菌  食物中毒  毒力基因  脉冲场凝胶电泳

英文关键词:Clostridium perfringens  food poisoning  virulence genes  pulsed-field gel electrophoresis

中文摘要:

      目的 分析一起疑似产气荚膜梭菌食物中毒事件的病原学。方法 利用荧光定量PCR方法对一起食物中毒患者的粪便进行产气荚膜梭菌基因的初筛, 根据荧光定量PCR初筛的提示结果进行细菌的分离培养, 对分离到的菌株进行质谱和生化鉴定、毒力基因检测和PFGE分析。结果 从5份食物中毒患者粪便中分离到5株携带肠毒素基因cpe的A型产气荚膜梭菌, 并且这5株菌的PFGE带型完全一致。结论 此次食物中毒致病因子为携带肠毒素基因cpe的A型产气荚膜梭菌, PFGE结果提示5株菌可能有同一来源。

英文摘要:

      Objective To analyze the etiology of a suspected food poisoning case of Clostridium perfringens. Methods The feces of patients with food poisoning were screened for Clostridium perfringens genes by fluorescence quantitative PCR method. The bacteria were isolated and cultured according to the results of the initial screening by fluorescence quantitative PCR, and the isolated strains were identified by mass spectrometry, biochemical identification, virulence gene detection and PFGE analysis. Results Totally 5 strains of Clostridium perfringens carrying enterotoxin gene cpe were isolated from the feces of 5 food poisoning patients, and the PFGE bands of the 5 strains were identical. Conclusion The pathogenic factor of this food poisoning was Clostridium perfringens type A, which carried the enterotoxin gene cpe. PFGE results suggested that 5 strains might have the same source.