彝良小草坝天麻急性毒性和诱变毒性作用的研究

Study on the acute toxicity and mutagenicity of Gastrodia elata in Yiliang Xiaocaoba

中文关键词:  小草坝天麻  急性毒性  LD50  Ames实验  微核  精子  诱变

英文关键词:Gastrodia elata  acute toxicity  LD50  Ames test  micronucleus  sperm  mutagenicity

中文摘要:

      目的 研究彝良小草坝天麻的急性毒性和诱变毒性作用。方法 通过小鼠急性经口毒性实验、Ames实验、小鼠骨髓细胞微核实验、小鼠精子畸形实验, 测定其急性毒性和遗传毒性作用。结果 小鼠急性经口毒性实验LD50＞10000 mg/kg?BW。Ames实验中, 各剂量组回复突变菌落数均未见明显增加(P＞0.05), 亦无剂量-反应关系。小鼠骨髓细胞微核实验中, 各剂量组微核细胞率与阴性对照组比较差异无显著性(P＞0.05)。小鼠精子畸变实验中, 各剂量组精子畸变率与阴性对照组比较差异无显著性(P＞0.05)。结论 该彝良小草坝天麻对昆明种小鼠属实际无毒, 在Ames实验中未呈现致突变作用, 未见诱导小鼠骨髓细胞微核形成的能力, 未见致小鼠精子畸变的能力。

英文摘要:

      Objective To study the acute toxicity and potential mutagenicity of Gastrodia elata in Yiliang Xiaocaoba. Methods Acute toxicity and genetic toxicity of mice were determined by mouth toxicity test, Ames test, micronucleus test of mouse bone marrow cell and sperm abnormality test. Results In mouse acute oral toxicity test, LD50 was higher than 10000 mg/kg?BW. In the Ames test, there was no significant increase in the number of revertant colonies in each dose group (P＞0.05), and no dose-response relationship. In the micronucleus test of mouse bone marrow cell, there was no significant difference in micronucleus cell rate between each dose group and the negative control group (P>0.05). In the sperm aberration test of mice, there was no significant difference in sperm aberration rate between each dose group and the negative control group (P>0.05). Conclusion The Yiliang xiaocaoba Gastrodia elata is actually non-toxic to Kunming mice, and shows no mutagenesis in Ames test, no ability to induce micronucleus formation of mouse bone marrow cell, and no ability to cause sperm distortion in mice.