超高效液相色谱-串联质谱法检测玉米油中的 黄曲霉毒素和玉米赤霉烯酮

Determination of aflatoxins and zearalenone in corn oil by ultra high performance liquid chromatography-tandem mass spectrometry

中文关键词:  黄曲霉毒素  玉米赤霉烯酮  玉米油  超高效液相色谱-串联质谱法

英文关键词:aflatoxins  zearalenone  corn oil  ultra high performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立超高效液相色谱-串联质谱法准确、快速检测玉米油中黄曲霉毒素(aflatoxins B1、B2、G1、G2)和玉米赤霉烯酮(zearalenone, ZEN)的含量, 了解市售玉米油中B1、B2、G1、G2、ZEN污染状况。方法 样品经乙腈:水(84:16, V:V)溶液超声离心提取后, 通过多功能固相萃取柱进行净化处理, 采用液相色谱-串联质谱法进行检测。结果 B1、B2、G1、G2的线性范围为0.1~20 μg/L, ZEN的线性范围为3.0~200 μg/L; 方法回收率为74.4%~98.6%, 相对标准偏差为3.9%~6.9%; B1、B2、G1、G2定量限为0.2 μg/L, ZEN的定量限为6.0 μg/L。市售的33份样品中, 均没有检出B1、B2、G1、G2; 玉米赤霉烯酮检出32份, 检出率为97.0%。结论 该方法操作简单快速、重现性好, 可用于玉米油中黄曲霉毒素和玉米赤霉烯酮的检测。

英文摘要:

      Objective To establish an analysis method for accurate and rapid determination of aflatoxins (B1, B2, G1, and G2) and zearalenone (ZEN) by ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and to elucidate the contamination situation of B1, B2, G1, G2 and ZEN in corn oil. Methods The sample was extracted by ultrasonic centrifugation of acetonitrile:water (84:16, V:V) solution, purified by multi-functional solid phase extraction column, and detected by UPLC-MS/MS. Results The linear ranges of B1, B2, G1 and G2 were 0.120 μg/L, and the linear range of ZEN was 3.0200 μg/L. The recoveris were 74.4%98.6%, and the relative standard deviations were 3.9%-6.9%. The limits of quantification for B1, B2, G1, and G2 were 0.2 μg/L, and the limit of quantification for ZEN was 6.0 μg/L. B1, B2, G1, and G2 were not detected in the samples, but ZEN were detected in 32 samples and the detection rate was 97.0%. Conclusion The method is simple, rapid, and reproducible, which can be used for the determination of aflatoxins and zearalenone in corn oil.