氨基酸分析仪检测婴幼儿奶粉中牛磺酸含量

Determination of taurine content in infant milk powder by amino acid analyzer

中文关键词:  婴幼儿奶粉  氨基酸分析仪  牛磺酸  含量

英文关键词:infant milk powder  amino acid analyzer  taurine  content

中文摘要:

      目的 建立一种氨基酸分析仪测定婴幼儿奶粉中牛磺酸含量的分析方法。方法 样品经0.01 mol/L盐酸溶液溶解并用106 g/L亚铁氰化钾溶液和219 g/L乙酸锌沉淀蛋白质后10000 r/min离心10 min取出, 精密取上清液置20 mL量瓶中, 用上样溶液(pH 2.2)定容后, 采用氨基酸分析仪钠系统进行程序洗脱, LCA K06/Na色谱柱进行分离, 茚三酮溶液衍生显色, 在570 nm波长下测得牛磺酸色谱峰, 用外标法峰面积定量, 保留时间定性。结果 牛磺酸在5.544~110.880 μg/mL浓度范围内有良好的线性关系(r=1.0000), 回收率为101.47%, 相对标准偏差为1.6%(n=9)。结论 该方法简单、便捷、准确性高, 适合于婴幼儿奶粉中牛磺酸的定量分析。

英文摘要:

      Objective To establish an method using amino acid analyzer for the determination of taurine in infant milk powder. Methods The sample was dissolved in 0.01 mol/L hydrochloric acid solution and precipitated with 106 g/L potassium ferrocyanide solution and 219 g/L zinc acetate. The protein was centrifuged at 10000 r/min for 10 min, and the supernatant was accurately taken in a 20 mL volumetric flask. After constant volume with a sample solution (pH 2.2), the program was eluted with an amino acid analyzer sodium system, separated by LCA K06/Na column, and colorimetrically derived from ninhydrin solution, and the taurine chromatographic peaks was measured at a wavelength of 570 nm, quantification of peak area by external standard method, retention time qualitative. Results Taurine had a good linear relationship in the concentration range of 5.544-110.880 g/mL (r=1.0000) with a recovery rate of 101.47% and a relative standard deviation of 1.6% (n=9). Conclusion This method is simple, convenient, accurate and suitable for the quantitative analysis of taurine in infant milk powder.