气相色谱-串联质谱法同时检测代用茶中的16种农药残留

Simultaneous determination of 16 kinds of pesticide residues in substitutional tea by gas chromatography-mass spectrometry

中文关键词:  代用茶  气相色谱-串联质谱法  农药残留  同时检测

英文关键词:substitutional tea  gas chromatography-mass spectrometry  pesticide residues  simultaneous detection

中文摘要:

      目的 建立气相色谱-串联质谱法同时检测代用茶中16种农药残留的分析方法。方法 代用茶样品中的农残经乙腈提取后, 用25 mg N-丙基乙二胺(primary secondary amine, PSA)、150 mg MgSO4净化, 引入分析保护剂补偿基质效应, 用气相色谱-串联质谱法测定其中16种农药残留。结果 16种农药成分在0.005~0.400 μg/mL范围内有良好线性关系, 相关系数均大于0.98; 检出限均在0.003~0.007 mg/kg之间, 定量限在0.011~0.022 mg/kg之间; 在3个添加水平下(0.04、0.10和0.40 mg/kg)的平均回收率为80.2%~104%, 相对标准偏差(n=6)为3.4%~12.4%。结论 本方法样品处理简单快速, 灵敏度和选择性高, 重复性好, 适用于代用茶中多种农药残留的同时检测。

英文摘要:

      Objective To establish a method for simultaneous detection of 16 kinds of pesticide residues in substitutional tea by gas chromatography-mass spectrometry. Methods Substitutional tea samples were extracted by acetonitrile and then purified by 25 mg primary secondary amine (PSA) and 150 mg MgSO4. Analyte protectants were added to compensate the matrix effect. The gas chromatography-mass spectrometry was used to determine 16 kinds of pesticide residues in the samples. Results The calibration curves for 16 kinds of pesticide residues were linear within the range from 0.005 to 0.400 μg/mL with the correlation coefficients all greater than 0.98. The limits of detection were from 0.003 to 0.007 mg/kg and the limits of quantitation were from 0.011 to 0.022 mg/kg. The average recoveries obtained from 3 spiked concentrations (0.04, 0.10 and 0.40 mg/kg) were from 80.2% to 104% with relative standard deviations (RSDs, n=6) ranging from 3.4% to 12.4%. Conclusions This method has simple and rapid sample processing, high sensitivity and selectivity, and good repeatability, which is suitable for simultaneous detection of multiple pesticide residues in different substitutional tea samples.