基因芯片法与常规检测法在食源性疾病中的应用对比分析

Comparative analysis of application of gene chip method and routine detection method in foodborne diseases

中文关键词:  食源性疾病  病原菌  基因芯片  培养法

英文关键词:foodborne disease  pathogen  gene chip  culture method

中文摘要:

      目的 对比分析基因芯片法与常规检测法在食源性疾病中的应用效果。方法 收集2016年4月到2018年8月收治的诊断为食源性疾病的患者98例为研究对象, 收集患者的新鲜粪便, 根据检测方法不同分为对照组与观察组, 观察组应用基因芯片法进行病原菌的检测, 对照组则应用常规培养法进行病原菌的检测。,观察比较两组的病原菌检测阳性率和检测时间等相关指标。结果 在98例患者中, 观察组采用基因芯片法检测出病原菌58例(副溶血弧菌9例, 痢疾杆菌32例, 沙门菌10例, 大肠埃希菌2例, 金黄色葡萄球菌5例), 病原菌检出率为58.2%。对照组采用常规培养法检测出病原菌32例(副溶血弧菌5例, 痢疾杆菌19例, 沙门菌4例, 金黄色葡萄球菌4例), 病原菌检出率为32.7%。基因芯片法和培养法对病原菌、痢疾杆菌的检出率差异存在统计学意义(P＜0.05)。观察组采用基因芯片法用于食源性疾病患者的病原菌检测时间明显短于常规培养法, 差异有统计学意义(P＜0.05)。结论 基因芯片技术能够快速检出食源性疾病病原菌, 可以替代常规培养法对病原菌进行检测, 在食源性疾病的早期诊断和治疗中具有重要的临床价值。

英文摘要:

      Objective To compare and analyze the effects of gene chip and conventional detection in foodborne diseases. Methods A total of 98 patients diagnosed with foodborne diseases were collected as study objects from April 2016 to August 2018. Fresh feces of patients were collected and divided into control group and observation group according to different detection methods. The observation group was tested for pathogens by gene chip method, while the control group was tested for pathogens by conventional culture method. The positive detection rate, detection time and other related indicators were observed and compared between the 2 groups. Results Among the 98 patients, the observation group detected 58 pathogens by gene chip method (9 cases of Vibrio parahaemolyticus, 32 cases of Shigella castellani, 10 cases of Salmonella, 2 cases of Escherichia coli, 5 cases of Staphylococcus aureus). The detection rate of pathogens was 58.2%. In the control group, 32 cases of pathogens were detected by routine culture method (5 cases of Vibrio parahaemolyticus, 19 cases of Shigella castellani, 4 cases of Salmonella and 4 cases of Staphylococcus aureus), and the detection rate of pathogenic bacteria was 32.7%. There was a statistically significant difference in the detection rate of pathogenic bacteria and dysentery bacilli by gene chip method and culture method (P<0.05). The detection time of the pathogens in the observation group using the gene chip method for foodborne diseases was significantly shorter than that of the conventional culture method, and the difference was statistically significant (P<0.05). Conclusion Gene chip technology can quickly detect the pathogen of foodborne disease, and can replace the conventional culture method to detect the pathogen. It has important clinical value in the early diagnosis and treatment of foodborne disease.