| 闫协民,何 蕾,周春霞,洪鹏志,孙省利,李承勇.基于Au纳米团簇荧光猝灭特性检测酪氨酸酶活性[J].食品安全质量检测学报,2019,10(21):7278-7282 |
| 基于Au纳米团簇荧光猝灭特性检测酪氨酸酶活性 |
| Detection of tyrosinase activity based on fluorescence quenching of Au nanoclusters |
| 投稿时间：2019-04-16 修订日期：2019-10-30 |
| DOI： |
| 中文关键词: 酪氨酸酶活性 Au纳米团簇 荧光猝灭 酪氨酸酶活 |
| 英文关键词:tyrosinase activity Au nanoclusters fluorescence quenching tyrosinase activity |
| 基金项目:国家自然科学基金(21874029)、广东省扬帆计划项目(201635018)、广东省特支计划项目(2017TQ04N706)、广东省科技攻关项目(2016A020210114) |
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| 中文摘要: |
| 目的 建立基于Au纳米团簇荧光猝灭特性检测酪氨酸酶(tyrosinase, TYR)活性的方法。方法 以谷胱甘肽为模板合成Au纳米团簇(Au nanoclusters AuNCs), 通过荧光猝灭检测TYR活性。结果 酪氨酸酶活力范围在11.55~69.30 U/mL时, 荧光猝灭减半时间(quenching time of half fluorescence intensity, QTHFI)与酪氨酸酶活力线性相关, r2为0.98, 检出限为1.28 U/mL. 结论 利用Au纳米团簇荧光特性检测酪氨酸酶活力的新方法可行。 |
| 英文摘要: |
| Objective To establish a method of detection of tyrosinase (TYR) activity was researched. Methods AuNCs was synthesized using glutathione (GSH) as the template, and TYR activity was detected by fluorescence quenching. Results There is a good linear relation between the quenching time of half fluorescence intensity (QTHFI) and tyrosinase activity ranging in 11.55?69.30 U/mL, r2 reached 0.98, the detection limit is 1.28 U/mL. Conclusion The method for detecting tyrosinase activity by the fluorescence characteristics of Au nanoclusters is feasible. |
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