大连地区输欧水产企业加工用水中微生物的 检测分析

Detection and analysis of microorganism in processing water of aquatic enterprises exported to Europe in Dalian area

中文关键词:  输欧水产企业  加工用水  微生物检测

英文关键词:aquatic enterprises exported to the European Union  processing water  microbiological testing

中文摘要:

      目的 针对大连地区输欧水产企业加工用水进行微生物监测, 调查其水质是否存在微生物污染风险。方法 依据欧盟新水质指令98/83/EEC的要求, 在50份水样中分别对细菌总数(22 ℃和37 ℃)、大肠埃希氏菌、肠球菌和铜绿假单胞菌进行检测, 对水样中的风险菌进行分离培养及生化鉴定, 并通过大肠埃希氏菌ydiJ基因和肠球菌ddl基因合成特异性引物及探针进行分子生物学方法的验证。结果 2种方法针对风险菌检出阳性结果一致。试验结果发现包含细菌总数(22 ℃和37 ℃)、大肠埃希氏菌和肠球菌在内的微生物指标不合格率为20%, 部分输欧水产企业没有达到欧盟加工用水的要求。结论 要充分认清水产企业加工用水在水产品加工环节中的重要地位, 做到对各个加工环节进行微生物风险监控, 以保证输欧水产品的质量安全。

英文摘要:

      Objective To carry out microbial monitoring of processing water used in aquatic production enterprises in Dalian, and investigate whether there is microbial contamination risk in water quality. Methods According to the requirements of the EU New Water Quality Directive 98/83/EEC, the total number of bacteria (22 °C and 37 °C), Escherichia coli, Enterococcus and Pseudomonas aeruginosa were detected in 50 water samples, respectively. The risk bacteria in the sample were isolated, cultured and biochemically identified, and verified by molecular biological methods by synthesizing specific primers and probes of Escherichia coli ydiJ gene and Enterococcus ddl gene. Results The 2 methods were consistent with the positive results for the risk bacteria. The test results showed that the microbiological index unqualified rate including the total number of bacteria (22 °C and 37 °C), Escherichia coli and Enterococcus was 20%, and some European aquatic products companies did not meet the requirements of EU processing water. Conclusion It is necessary to fully understand the important role of processing water in aquatic enterprises in the processing of aquatic products, and to carry out microbiological risk monitoring on various processing links to ensure the quality and safety of aquatic products exported to Europe.