固相萃取-超高效液相色谱-串联质谱法快速测定鸡蛋和鸡肉中的7种性激素药物残留量

Rapid determination of 7 kinds of hormones residues in eggs and chicken by solid phase extraction coupled with ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  Oasis PRIME HLB固相萃柱  超高效液相色谱-串联质谱法  性激素残留  鸡蛋  鸡肉

英文关键词:Oasis PRIME HLB solid phase extraction column  ultra performance liquid chromatography- tandem mass spectrometry  hormones residues  egg  chicken

中文摘要:

      目的 建立同时测定鸡蛋和鸡肉中7种性激素类药物残留的快速检测方法。方法 鸡蛋和鸡肉中的性激素类药物经乙腈-水溶液(80:20, V:V)提取后, Oasis PRiME HLB固相萃取净化, UPLC-MS/MS法检测, 基质匹配外标法定量。结果 该方法具有良好的准确度和精密度, 在鸡蛋和鸡肉中添加回收率范围分别为78.7%~101.7%和77.9%~104.3%, 相对标准偏差(relative standard deviation, RSD)低于8.7%, 检出限(limit of detection, LOD, S/N=3)和定量限(limit of quantitation, LOQ, S/N=10)分别为0.02~0.5 μg/kg和0.08~2.0 μg/kg。结论 本方法简便快速, 灵敏可靠, 适用于鸡蛋和鸡肉中性激素类药物残留高通量快速检测分析。

英文摘要:

      Objective To establish a method for the simultaneous determination of 7 kinds of hormones residues in eggs and chicken. Methods The hormones residues in eggs and chicken were extracted by cetonitrile/water (80:20, V:V). The extracts were purified by the Oasis PRiME HLB solid phase extraction, then analyzed by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), and the matrix-matching external standard method was used to quantify. Results The method had good accuracy and precision, and the recoveries of egg and chicken were 78.7%-101.7% and 77.9%-104.3%, respectively, with the relative standard deviations (RSDs) of lower than 8.7%. The limits of detection (LODs, S/N≥3) and quantification (LOQs, S/N≥10) were 0.02-0.5 μg/kg and 0.8-2.0 μg/kg, respectively. Conclusion The method is simple, fast, sensitive and reliable, which is suitable for the rapid determination of hormones residues in eggs and chicken.