电泳法在食品过敏原检测中的应用

Application of electrophoresis in the detection of food allergens

中文关键词:  食品  过敏原  电泳法  毛细管电泳法

英文关键词:food  allergens  electrophoresis  capillary electrophoresis

中文摘要:

      本文简要介绍了食品过敏原的检测特点和难点, 比较了目前常用的聚合酶链式反应法、环介导等温扩增法、酶联免疫法、液相色谱法和液相色谱质谱法等检测方法的优劣势。主要介绍了电泳技术的特点, 总结了经典电泳技术在食品过敏原分析上的应用现状。详细介绍了近年来毛细管电泳技术在食品过敏原检测研究方面取得的进展, 列举了区带毛细管电泳法、亲和毛细管电泳法、凝胶毛细管电泳法、动态涂层毛细管电泳法和芯片毛细管电泳法在致敏蛋白分析方面的应用, 并对电泳法在食品过敏原分析中的发展趋势进行了展望。

英文摘要:

      This paper briefly introduced the detection characteristics and difficulties of food allergens and compared the advantages and disadvantages of the commonly used detection methods such as polymerase chain reaction (PCR), loop mediated isothermal amplification (LAMP), enzyme linked immunosorbent assay (ELISA), liquid chromatography (LC) and liquid chromatography mass spectrometry (LC-MS). At the same time, this article mainly introduced the characteristics of electrophoresis technology, summarized the application status of classical electrophoresis technology in food allergen analysis, introduced the progress of capillary electrophoresis in the detection of food allergens in recent years in detail, listed the advantages and disadvantages of capillary zone electrophoresis (CZE), affinity capillary electrophoresis (ACE), capillary gel electrophoresis (CGE), dynamic coated capillary electrophoresis (DCCE) and chip capillary electrophoresis (Chip-CE) in the analysis of allergenic proteins, and prospected the development trend of electrophoresis in food allergen analysis.