离子色谱-脉冲安培检测法测定食品中的聚葡萄糖

Determination of polydextrose in foods by ion chromatography with pulsed amperometric detection

中文关键词:  离子色谱  聚葡萄糖  脉冲安培

英文关键词:ion chromatography  polydextrose  pulsed amperometric

中文摘要:

      目的 建立食品中添加的聚葡萄糖(polydextrose, PDX)的离子色谱-脉冲安培检测方法。方法 采用热水浸提聚葡萄糖, 超滤离心去除高分子量的干扰物, 滤液经酶解去除其他低聚糖的干扰, 采用Hamilton RCX-30-250/4.6色谱分离柱; 以含有A: 150 mmol/L氢氧化钠和B: 150 mmol/L氢氧化钠+500 mmol/L乙酸钠的淋洗液进行梯度淋洗, 采用三电位波形, 以同源标准物质建立标准曲线, 流速1.2 ml/min, 柱温32 ℃。结果 本方法在30~200 μg/g范围内, 线性良好, r2=0.9993。在检测固体样品时检出限为1.6699 mg/kg, 在检测液体样品时检出限为0.9555 mg/kg, 重复性测量相对标准偏差小于4.88%(n=6), 不同食品基质聚葡萄糖的平均加标回收率为95.06%~110.11%。结论 该方法灵敏度高, 准确性好, 结果稳定, 适用于不同食品中添加的聚葡萄糖的测定。

英文摘要:

      Objective To establish a method for the determination of polydextrose (PDX) added in foods by ion chromatography with pulsed amperometric detection. Methods The sample was extracted by hot water, the high molecular weight interferent was removed by ultrafiltration centrifugation, and the other oligosaccharides was removed by enzymatic hydrolysis. Hamilton RCX-30-250/4.6 column was used for separation. The mobile phase was with A: 150 mmol/L sodium hydroxide and B: 150 mmol/L sodium hydroxide+500 mmol/L sodium acetate eluent by gradient elution, three potential waveforms were used to establish the standard curve with homologous reference materials, flow rate was 1.2 mL/min, and column temperature was 32 ℃. Results The PDX had a good linearity in the concentration range of 30-200 μg/g (r2=0.9993). The limit of detection in solid sample was 1.6699 mg/kg, and in liquid sample was 0.9555 mg/kg. The recoveries were ranged from 95.06% to 110.11% for the PDX in different foods. The relative standard deviations (RSDs) were less than 4.88% (n=6). Conclusion This method is sensitive, accurate, stable, which is suitable for the determination of polyglucose in different foods.