不同来源氨基脲在刺参体内的富集和消除规律研究

Rules of accumulation and elimination of semicarbazide residue from different sources in Apostichopus japonicus

中文关键词:  氨基脲  呋喃西林  刺参  液相色谱-串联质谱法

英文关键词:semicarbazide  nitrofurazone  Apostichopus japonicus  high performance liquid chromatography tandem mass spectrometry

中文摘要:

      目的 分析刺参中低浓度氨基脲的残留分布特征, 明确不同来源引起的低浓度氨基脲的变化趋势。方法 采用超高效液相色谱-串联质谱法对不同来源氨基脲在刺参(Apostichopus japonicus)体内的富集和消除规律进行研究。结果 非呋喃西林源氨基脲, 1 d后氨基脲在刺参体壁的含量为0.57 μg/kg, 之后含量逐渐上升, 到3 d时含量度达到最大值1.00 μg/kg。富集实验共持续3 d, 按天计算平均富集速率, 分别为0.57、0.24、 0.19 μg/(kg?d), 第140 d时体壁未检出, 平均消除速率为0.0073 μg/(kg?d); 呋喃西林源氨基脲, 1 d后体壁的含量为0.52 μg/kg, 之后含量逐渐上升, 到3 d时, 含量达到最大1.00 μg/kg。富集实验共持续3 d, 按天计算平均富集速率, 分别为0.52、0.26、0.22 μg/(kg?d), 第160 d时体壁未检出, 平均消除速率为0.0064 μg/(kg?d)。跟踪监测至180 d时, 非呋喃西林源氨基脲和呋喃西林源氨基脲在刺参体壁内均未检出, 半衰期分别为1045.7 h和1224.2 h, 呋喃西林源大于非呋喃西林源。内脏的富集和消除速率相对较快, 但内脏中氨基脲残留量大于体壁, 所以降至未检出所需时间更长。结论 在投喂过呋喃西林或暴露于一定浓度氨基脲的刺参, 需经较长时间净化后才能降至未检出。

英文摘要:

      Objective To analyze the distribution characteristics of low residues of semicarbazide in Apostichopus japonicus, and clarify the variation trend of low concentration urea from different sources. Methods Rules of accumulation and elimination of semicarbazide residue from different sources in Apostichopus japonicus was studied by high performance liquid chromatography tandem mass spectrometry method (HPLC-MS/MS). Results Non-nitrofurazone source semicarbazide, the content in the body wall was 0.57 μg/kg after 1 d, and then increased gradually, reaching the maximum value of 1.00 μg/kg at 3 d, and the average enrichment rate was 0.57, 0.24 and 0.19 g/(kg?d), respectively. At the 140 th day, it was not detected in body wall, and the average removal rate was 0.0073 g/(kg?d). Nitrofurazone source semicarbazide, the content was 0.52 μg/kg after 1 d, and then increased gradually, reaching 1.00 μg/kg at 3 d, and the average enrichment rate was 0.52, 0.26, 0.22 g/(kg?d), respectively. At the 160 th day, it was not detected in body wall, and the average removal rate was 0.0064 μg/(kg?d). At 180 d, 2 source semicarbazide were not detected in the body wall of Apostichopus japonicus, with half-lives of 1045.7 h and 1224.2 h, respectively. The semicarbazide caused by nitrofurazone longer than direct exposure. The enrichment and elimination rate of semicarbazide in viscera was relatively fast, but the residue of semicarbazide in viscera was larger than the body wall, so it took longer time to decrease to undetected. Conclusion Apostichopus japonicus fed with nitrofurazone or grown in the seawater at a certain content of semicarbazide can be decreased to undetected only after purified for a long time.