高效液相色谱法同时测定保健品中15种激素

Determination of 15 kinds of hormones in health products by high performance liquid chromatography

中文关键词:  高效液相色谱法  保健品  激素  样品前处理  二极管阵列检测器

英文关键词:high performance liquid chromatography  health products  hormones  sample pretreatment  diode array detector

中文摘要:

      目的 建立高效液相色谱法同时测定保健品中雌三醇、雌二醇、雌酮、己烯雌酚、泼尼松龙、氢化可的松、可的松、倍他米松、地塞米松、曲安奈德、醋酸泼尼松、睾丸酮、甲基睾丸酮、氯倍他索丙酸酯、黄体酮等15种激素的分析方法。方法 用甲醇直接溶解提取, C18固相萃取柱净化, 以乙腈-水为流动相进行梯度洗脱, 流量为1.0 mL/min, 采用二极管阵列检测器双波长检测, 波长分别为204、245 nm。结果 15种激素的线性范围为0.1~5 mg/L, 相关系数r>0.99, 添加回收率为85.0%~105%之间, 相对标准偏差<5%, 定量限 0.05 mg/kg。结论 该方法简便、快捷、准确, 适用于保健品中15种激素的测定。

英文摘要:

      Objective To establish a method for determination of 15 kinds of hormones in health products by high performance liquid chromatography. Method Health products sample were extracted in methanol, purified by C18 column, acetonitrile/water was used as mobile phase at the flow rate of 1.0 mL/min and detected at the wavelength of 204, 245 nm. Results There were good linear relationships over the range of 0.1-5 mg/L for all the hormones (r>0.99). The recoveries were ranged from 85.0% to 105%. The relative standard deviations were less than 5%. The limits of quantitation were 0.05 mg/kg. Conclusion This method is simple, rapid, and accurate, which is applicable to simultaneous determination of 15 kinds of hormones in health products.