贮藏期影响不同品种马铃薯龙葵碱风险的主要环境因素

Main environmental factors of solanum during storage for different potato varieties

中文关键词:  贮藏因素  马铃薯  龙葵碱  α-茄碱  α-卡茄碱

英文关键词:storage factors  potato, solanine  α- solanine  α-chaconine

中文摘要:

      目的 探究影响不同品种马铃薯在不同贮藏条件下龙葵碱含量的主要环境因素, 探讨马铃薯贮藏过程中龙葵碱含量的变化规律。方法 样品经乙酸-乙醇( 1:10, V:V)混合溶剂提取后, 用甲醇复溶并稀释后, 采用高效液相色谱－三重四级杆串联质谱(high performance liquid chromatography-triple quadrupole tandem mass spectrometry, HPLC-QQQ-MS)测定马铃薯块茎中龙葵碱含量, 通过正交试验研究8个品种的马铃薯块茎在不同贮藏温度(5、15、25 ℃)、相对湿度(55%、70%、85%)、贮藏时间(8、16、24 d)下龙葵碱含量的变化情况。结果 结果表明影响大西洋、新大坪、荷兰15号、夏波蒂与陇薯3号龙葵碱的贮藏主要环境因素为相对湿度; 威芋5号与冀张12号的主要环境因素为温度; 青薯9号品种的贮藏主要环境因素为时间。结论 建议针对不同品种马铃薯重点控制不同环境因素, 保障龙葵碱含量安全, 其中青薯9号不宜长期贮藏。

英文摘要:

      Objective To explore the changes of solanine content in different potato varieties, and investigate the main environmental factors affecting the solanine during storage. Methods The samples were extracted with acetic acid: ethanol (1:10, V:V) mixed solvent, reconstituted with methanol and diluted, and then determined by high performance liquid chromatography-triple quadrupole tandem mass spectrometry (HPLC-QQQ-MS) to determine the content of solanine in potato tubers. The content of potato solanine was studied by orthogonal experiment at different storage temperatures (5, 15, 25 ℃), relative humidity (55%, 70%, 85%), and storage time (8, 16, 24 d). Results The results showed that the main environmental factor of the storage of Daxiyang, Xindaping, Helan No.15, Xiabodi and Qishu No.3 was relative humidity; the main environmental factor of Weiyu No.5 and Jizhang No.12 was temperature; and the main environmental factor for the storage of Qingshu No.9 was time. Conclusion To guarantee the safety content of solanine, it is suggested that different varieties should be concerned more about their own main environmental factors during storage. In addition, Qingshu No. 9 is not suitable for long-term storage.