2018年巧克力中沙门氏菌检测能力验证结果与分析

Validation and analysis of Salmonella detection ability in chocolate in 2018

中文关键词:  沙门氏菌  能力验证  分离  血清学鉴定

英文关键词:Salmonella  proficiency verification  separate  serologial identification

中文摘要:

      目的 参加中国食品药品检定研究院组织的NIFDC-PT-135巧克力中沙门氏菌检验能力验证, 提高实验室的检测技术能力与水平。方法 按照盲样作业指导书的要求对样品进行前处理后, 依据GB 4789.4-2016《食品安全国家标准 食品微生物学检验 沙门氏菌检验》中的方法, 对3份样品进行沙门氏菌的分离和血清学鉴定, 对分离出的可疑菌落用全自动微生物鉴定系统进行鉴定。结果 CODE0109样品中检出鼠伤寒沙门氏菌、CODE0143样品中检出肠沙门双相亚利桑那亚种(沙门氏菌Ⅲb), CODE0835样品中未检出沙门氏菌。本实验室顺利完成考核, 测定结果与考核结果一致。结论 本次能力验证检出的肠沙门双相亚利桑那亚种在沙门氏菌显色平板上出现蓝色菌落, 而以往则为紫红色菌落, 因此在检测中应引起重视。通过此次能力验证, 本实验室的沙门氏菌检测技术水平得到了有效验证和提高。

英文摘要:

      Objective To improve the detection ability and level of Salmonella of laboratories, participating in the validation of Salmonella in NIFDC-PT-135 chocolate organized by National Institutes for Food and Drug Control. Methods After pre-treatment of the samples according to the requirements of the blind sample operation guide, according to the method in GB 4789.4-2016 National food safety standard-Food microbiology test-Salmonella test, the isolation and serological identification of Salmonella in 3 samples were performed. Suspicious colonies were identified using a fully automated microbial identification system. Results Salmonella typhimurium was detected in CODE0109 sample, intestinal Salmonella biphasic Arizona subspecies (Salmonella IIIb) was detected in CODE0143 sample and Salmonella was not detected in CODE0835 sample. The laboratory successfully completed the assessment, and the measurement results were consistent with the assessment results. Conclusion The intestinal Salmonella biphasic Arizona subspecies detected by this proficiency test showed blue colonies on the Salmonella color-developing plate, but in the past it was a purple-red colony, so it should be paid attention to in the detection. Through this proficiency test, the laboratory's detection level of Salmonella has been effectively verified and improved.