QuEChERS-超高效液相色谱法快速测定果蔬中4种植物激素残留

Rapid determination of 4 kinds of phytohormone residues in fruits and vegetables by QuEChERS-ultra performance liquid chromatography

中文关键词:  QuEChERS  超高效液相色谱法  植物激素  果蔬  残留

英文关键词:QuEChERS  ultra performance liquid chromatography  phytohormone  fruit and vegetable  residue

中文摘要:

      目的 建立超高效液相色谱法快速测定果蔬中4种植物激素残留方法。方法 样品经甲醇提取后, 提取液采用分散固相萃取吸附剂C18进行吸附净化, 用乙腈-0.02 mol/L乙酸铵溶液为流动相, Waters ACQUITY UPLC?BEH C18色谱柱分离进行液相色谱梯度洗脱, 二极管阵列检测器200~400 nm检测, 外标法定量。结果 4种植物激素在0.1~4.0 μg/mL范围内线性关系良好, 相关系数r≥0.9997, 其检出限为0.006~0.032 mg/kg, 样品的加标回收率为83.8%~98.4%, 相对标准偏差为2.06%~3.89%。结论 该方法样品前处理简单, 快速高效, 重现性好, 准确度高, 适用于果蔬中4种植物激素残留的检测。

英文摘要:

      Objective To establish a rapid method for the determination of 4 kinds of phytohormone residues in fruits and vegetables by ultra performance liquid chromatography (UPLC). Methods After the sample was extracted with methanol, the extracted liquid was adsorbed and purified by dispersed solid phase extraction adsorbent C18. The mobile phase was acetonitrile-0.02 mol/L ammonium acetate solution. The sample was separated by Waters ACQUITY UPLC?BEH C18 column for gradient elution, and was detected by diode array detector at 200~400nm and quantified by external standard method. Results Four kinds of phytohormones had good linear relationships in the range of 0.1?4.0 μg/mL, and the correlation coefficients (r) was 0.9997. The limits of detection were 0.006~0.032 mg/kg. The average recoveries of 4 kinds of phytohormones were 83.8%?98.4%, with the relative standard deviations of 2.06%?3.89%. Conclusion This method is simple, rapid, efficient, reproducible and accurate, which is suitable for the detection of 4 kinds of plant hormones residues in fruits and vegetables.