东莞市区肉菜市场熟肉制品中金黄色葡萄球菌污染溯源分析

Molecular traceability of Staphylococcus aureus in cooked meat products in Dongguan food market

中文关键词:  金黄色葡萄球菌  熟肉制品  分子溯源  核糖体基因分型

英文关键词:Staphylococcus aureus  meat products  molecular traceability  ribotyping

中文摘要:

      目的 优化熟肉制品中金黄色葡萄球菌的快速污染溯源分析方法。方法 对熟肉制品及其加工环境进行采样, 应用3M PerifilmTM金黄色葡萄球菌快速测试片对样品定量检测, 对分离出的阳性菌株运用全自动微生物基因指纹鉴定系统RiboPrinter, 结合EcoRI酶, 对其进行分子溯源。结果 168个样品中分离出的7株菌共分为2种核糖体基因型, 其中6株为同一核糖体条带类型, 显示来源于相同克隆株。结论 该方法全程只需3 d时间, 快速稳定, 可满足监管快速检测鉴定、分型溯源的要求。RiboPrinter的优势在于快速溯源, 进行种的鉴定时应结合其他手段来进行确认。

英文摘要:

      Objective To optimize the rapid method for source analysis of Staphylococcus aureus in cooked meat product. Methods Staphylococcus aureus were collected from the meat products and the processing environment by using the 3M PerifilmTM. The isolations were source analyzed by the automated ribotyping system using the restriction enzymes EcoRI. Results The 7 strains isolated from 168 samples were divided into 2 ribosome genotypes, and 6 of which were of the same ribosome band type, indicating that they were derived from the same clone. Conclusion This method only needs 3 days for the whole process, which is fast and stable, and can meet the requirements of supervision, rapid detection, identificationand typing traceability. The advantage of RiboPrinter is the rapid traceability. The identification of the species should be combined with other means.