碳源对鼠李糖乳杆菌生长特性的影响研究

Distinguish Different Strains of Lactobacillus rhamnosus using Growth Curves

中文关键词:  鼠李糖乳杆菌  生长特性  不同碳源  差异分析  主成分分析

英文关键词:Lactobacillus rhamnosus  growth characteristics  different carbon sources  difference analysis  principal component analysis

中文摘要:

      目的 研究不同鼠李糖乳杆菌菌株对不同碳源的利用及生长特性。方法 在600 nm波长处测定12株鼠李糖乳杆菌在不同碳源条件下的吸光度, 并采用差异分析法和分类主成分分析法对其进行分析。结果 利用特定时间点的差异分析, 在葡萄糖作为主要碳源时, 各菌株之间的生长情况均没有显著差异(P>0.05)。12株菌在乳糖作为主要碳源时可以分为4个生长型, 在蔗糖作为主要碳源时可以分为5个生长型。主成分分类法在葡萄糖作为主要碳源时, 能将12株菌分为7个生长型, 在乳糖作为主要碳源时可分为8个生长型, 在蔗糖作为主要碳源时可分为10个类型。结论 不同鼠李糖乳杆菌菌株对不同碳源的利用存在差异, 表明菌株在碳源利用上具有菌株特异性, 同时不同菌株利用碳源的特异性会受到碳源种类的影响。

英文摘要:

      Objective To study the utilization of different carbon sources and growth characteristics of different Lactobacillus rhamnosus strains. Methods The absorbance of 12 strains under different carbon sources was measured at a wavelength of 600 nm, and analyzed by difference analysis and principal component analysis (PCA) classification. Results Using the difference analysis at specific time, there was no significant difference in growth between the strains with the main carbon source of glucose (P>0.05). Totally 12 strains could be divided into 4 growth types with the main carbon source of lactose, 5 growth types with the main carbon source of sucrose. Using the PCA classification, 12 strains could be divided into 7 growth types with the main carbon source of glucose, 8 growth types with the main carbon source of lactose and 10 growth types with the main carbon source of sucrose. Conclusion Different L. rhamnosus strains utilize different carbon sources differently, indicating that the strains have strain specificity in carbon sources utilization, and the specificity of different strains using carbon sources can be affected by the types of carbon sources.