溶氧量及pH对太平洋牡蛎类A型组织血型抗原表达的影响研究

Effects of dissolved oxygen and pH on the expression of A type HBGA-like in pacific oyster

中文关键词:  太平洋牡蛎  类组织血型抗原  溶氧量  pH  表达变化

英文关键词:pacific oyster  HBGAs-like  dissolved oxygen  pH  expression change

中文摘要:

      目的 研究环境因素溶氧量及pH对太平洋牡蛎类A型组织血型抗原表达的影响。方法 分离提取太平洋牡蛎中的类组织血型抗原, 利用人体组织血型抗原抗体进行分型, 采用ELISA方法对类A型组织血型抗原进行检测, 计算P/N值, 通过人工模拟改变牡蛎养殖条件海水溶氧量及pH, 检测太平洋牡蛎内脏及鳃中类A型组织血型抗原的含量变化, 分析溶氧量及pH对类A型组织血型抗原的影响。结果 通过对牡蛎不同组织的类组织血型抗原进行分型, 选定了牡蛎内脏及鰓中类A型组织血型抗原作为后续实验的主要研究型别, 人工模型实验结果显示高溶氧量利于牡蛎内脏中类A型组织血型抗原的表达, 高pH利于牡蛎鳃中类A型组织血型抗原的表达。结论 实验结果与诺如病毒疫情暴发期为冬春季节的现象吻合, 表明了诺如病毒疫情暴发的季节性与牡蛎类A型组织血型抗原的表达量存在一定的相关性。

英文摘要:

      Objective To study the effects of dissolved oxygen and pH on the expression of A type HBGA-like in pacific oysters. Methods The HBGAs-like in the pacific oysters were isolated and extracted, and the human HBGAs were used for typing. The A type HBGAs was detected by ELISA method, and the P/N value was caculated. The content changes of A type HBGA-like in the guts and gills of pacific oysters were detected by changing the dissolved oxygen content and pH of seawater under artificial simulation conditions. Results The HBGA-like of different tissues of oysters were classified, and the A type HBGA-like of gut and gill were selected as the main research type of subsequent experiments; the artificial simulation experiment results showed that the high pH could improve the expression of A type HBGA-like in pacific oysters gills, and the high dissolved oxygen can also improve the expression of A-type HBGA-like in pacific oysters guts. Conclusion The results were consistent with the phenomenon that the norovirus outbreak usually occur in winter and spring, indicating that the seasonality of norovirus outbreak was correlated with the expression of A type HBGAs-like in oyster.