白酒中邻苯二甲酸二(2-乙基)己酯荧光探针测定方法的构建

Establishment of new fluorescence probe method for detection of di(2-ethylhexyl)phthalate in white spirit

中文关键词:  芘  邻苯二甲酸二(2-乙基)己酯  荧光探针法

英文关键词:pyrene  di(2-ethylhexyl)phthalate  fluorescent probe method

中文摘要:

      目的 构建一种塑化剂邻苯二甲酸二(2-乙基) 己酯(di(2-ethylhexyl)phthalate, DEHP)含量测定的荧光探针检测方法。方法 基于芘分子满足激基缔合物的要求, 本文选择以芘为荧光探针, 设计合成了芘-聚氧乙烯蓖麻油体系(芘-EL体系)。结果 芘荧光探针在该体系中结合DEHP后发生荧光猝灭, 且荧光强度的降低与DEHP的浓度之间存在线性关系。荧光探针检测的最优条件为波长为374 nm, 400 μmol/L芘-EL体系, 相互作用时间30 min。该方法对DEHP测定的线性范围为5~30 μmol/L, 线性方程为△F=-23.153+24.179C, 线性相关系数r=0.9929, 检出限为0.063 μmol/L, 相对标准偏差为4.43%(n=6)。结论 本方法具有可视化、成本低、高灵敏度、高选择性、操作简便等优点, 适用于白酒中DEHP的测定。

英文摘要:

      Objective To develop a method for the determination of the content of the plasticizer phthalate (di(2-ethylhexyl)phthalate, DEHP) by fluorescence probe detection. Methods Based on the requirement that the pyrene molecule satisfies the radical connective compound, pyrene was selected as the fluorescent probe in this paper, and pyrene-polyoxyethylene castor oil system (pyrene-EL system) was designed and synthesized. Results The fluorescence quenching of pyrene fluorescence probe combined with diethylene phthalate(2-ethyl) hexyl ester (DEHP) occurred in the system, and a linear relationship existed between the reduction of fluorescence intensity and the concentration of DEHP. The optimal condition for fluorescence probe detection was a pyrene-EL system with a wavelength of 374 nm and interaction time of 30 min. The linear range of the method for the determination of DEHP was 5-30 μmol/L, the linear equation was ?F=-23.153+24.179C (r=0.9929), and the limit of detection was 0.063 μmol/L, the relative standard deviation was 4.43% (n=6). Conclusion The method has the advantages of visualization, low cost, high sensitivity, high selectivity and simple operation, which is suitable for the determination of DEHP in liquor.