优化生活饮用水中微生物检测的处理方法

Optimization of treatment method for microbial detection in drinking water

中文关键词:  生活饮用水  总大肠菌群  耐热大肠菌群  大肠埃希氏菌  滤膜法  稀释方法  能力验证

英文关键词:drinking water  total coliform  thermostable coliform  Escherichia coli  membrane filter technique  dilution method  proficiency testing

中文摘要:

      目的 优化生活饮用水微生物检测中总大肠菌群、耐热大肠菌群、大肠埃希氏菌滤膜法的稀释及过滤方法, 并验证方法可行性。方法 待测水样稀释1倍, 旋转加入稀释液过滤, 再依据国家标准GB/T 5750.12-2006 《生活饮用水标准检验方法 微生物指标》滤膜法的条件, 培养、验证; 同时按照该方法处理能力验证待测水样, 检验并上报结果。结果 总大肠菌、耐热大肠菌、大肠埃希氏菌菌落在滤膜上生长均匀, 可计数, 能力验证结果|Z|均小于2。结论 通过能力验证证实本实验稀释方法合理, 过滤手段有效, 适用于复杂水样总大肠菌群、耐热大肠菌群、大肠埃希氏菌的检测。

英文摘要:

      Objective To optimize the dilution and filtration methods of total coliform, heat-resistant coliform and Escherichia coli in the detection of microorganisms in drinking water, and verify the feasibility of the method. Methods The water sample to be tested was diluted 1 time, added to the dilution solution by rotation, and then cultured and verified according to the conditions of the national standard GB/T 5750.12-2006 Standard test method for drinking water standards microbiological index. The processing capacity of this method was performed to verify the water sample, and the results were reported. Results Total coliform, heat-resistant coliform and Escherichia coli colonies grew uniformly on the filter membrane and could be counted. The results of capacity verification showed that |Z| were all less than 2. Conclusion Through the verification of capability, it is proved that the dilution method of this experiment is reasonable and the filtration method is effective. It is suitable for the detection of total coliform bacteria, heat-resistant coliforms and Escherichia coli in complex water samples.