高效液相色谱法检测鸡蛋中泰乐菌素残留

Determination of tylosin residues in egg by high performance liquid chromatography

中文关键词:  鸡蛋  高效液相色谱法  泰乐菌素  残留

英文关键词:egg  high performance liquid chromatography  tylosin  residues

中文摘要:

      目的 建立一种鸡蛋中泰乐菌素残留的高效液相色谱 (high performance liquid chromatography, HPLC)检测方法。方法 样品中的泰乐菌素残留, 经二氯甲烷提取, 离心分层净化, 高效液相色谱法测定, 外标法定量。结果 本方法在20 min内完成泰乐菌素的分离。泰乐菌素在100、200和500 μg/L添加水平的回收率为74.59%~102.03%, 相对标准偏差小于10%(n=6), 方法检出限为50 μg/kg, 定量限为100 μg/kg。结论 该方法快速、准确, 适合测定泰乐菌素残留。

英文摘要:

      Objective To establish a method for determination of tylosin residues in egg by high performance liquid chromatography (HPLC). Methods The residue of tylosin in the sample was extracted by dichloromethane, purified by centrifugation and stratification, determined by high performance liquid chromatography, and quantified by external standard method. Results This method could complete the separation of tylosin within 20 min. The recoveries for tylosin residues with 3 spiked levels of 100, 200 and 500 μg/kg were ranged from 74.59% to 102.03%, with the relative standard deviations (RSDs) less than 10% (n=6). The limit of detection was 50 g/kg, and the limit of quantitation was 100 g/kg. Conclusion This method is rapid and accurate, which is suitable for determination of tylosin residues in egg.