QuEChERS技术结合高效液相色谱-串联质谱法快速测定动物源性食品中克伦特罗的残留量

Rapid determination of clenbuterol residues in animal-derived foods by QuEChERS technique with high performance liquid chromatography-tandem mass spectrometry

中文关键词:  克伦特罗  高效液相色谱-串联质谱法  ProElut QuEChERS

英文关键词:clenbuterol  high performance liquid chromatography-tandem mass spectrometry  ProElut QuEChERS

中文摘要:

      目的 建立一种高效液相色谱-串联质谱法(high performance liquid chromatography-tandem mass spectrometry, HPLC-MS/MS)快速检测动物源性食品中克伦特罗残留量。方法 样品经β-葡萄糖醛甘酶酶解, 乙腈提取, 经过ProElut QuEChERS净化管净化。采用0.1%甲酸(A)和乙腈(B)作为流动相进行梯度洗脱, 用多反应监测模式(multiple reaction monitoring, MRM)对克伦特罗含量进行检测。结果 该方法可以在15 min内完成目标物的分离分析。克伦特罗在0.5、1、5 μg/kg添加水平的回收率为90.0%~94.3%, 相对标准偏差小于10%(n=6), 方法检出限为0.5 μg/kg。结论 该方法快速、准确、灵敏, 适合用于动物源性食品中克伦特罗的残留量的测定。

英文摘要:

      Objective To establish a method for determination of clenbuterol residues in animal-derived foods by QuEChERS technique with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods The sample was digested with β-glucuronide, extracted with acetonitrile, and purified by a ProElut QuEChERS purification tube. Gradient elution was carried out using 0.1% formic acid (A) and acetonitrile (B) as mobile phases, and clenbuterol content was detected by multiple reaction monitoring (MRM). Results The sample could be separated and analyzed within 15 min. The recoveries of clenbuterol at 0.5, 1, 5 μg/kg were 90.0%-94.3%, and the relative standard deviations were less than 10% (n=6). The detection limit of the method was 0.5 μg/kg. Conclusion The method is rapid, accurate and sensitive, which is suitable for the determination of clenbuterol residues in animal-derived foods.