柱前衍生高效液相色谱法测定功能饮料中牛磺酸含量的条件优化

Optimization of the method for determination of taurine in functional drinks by high performance liquid chromatography with precolumn derivatization

中文关键词:  牛磺酸  高效液相色谱法  功能饮料

英文关键词:taurine  high performance liquid chromatography  functional drinks

中文摘要:

      目的 优化柱前衍生高效液相色谱条件检测功能饮料中牛磺酸的含量。方法 采用配备紫外检测器的高效液相色谱仪, 利用C18反相色谱柱, 选择甲醇:乙酸钠=45:55(V:V)为流动相, 在流速为0.8 mL/min、标液与衍生试剂比例为1:1(V:V)、衍生时间为1 h时进行检测。结果 在1.0~200 μg/mL范围内, 牛磺酸的浓度与峰面积呈现良好的线性, 标准曲线方程是Y=34182X+14599, r2=0.9999, 优化后的方法回收率在96.83%~100.17%之间, 检出限是0.53 mg/100 g, 定量限为1.76 mg/100 g。结论 本方法高效、准确, 适用于能饮料中牛磺酸含量的检测, 为日常监管工作提供理论支持。

英文摘要:

      Objective To optimize the conditions of high performance liquid chromatography (HPLC) with precolumn derivatization for determination of taurine content in functional drinks. Methods The samples were determined by HPLC with ultraviolet detector, using C18 reversed phase as chromatograph column. The mobile phase was methanol and sodium acetate (45:55, V:V), with the flow rate of 0.8 mL/min, the ratio of standard solution and derivatization of 1:1, and the derivatization time of 1 h. Results When the concentration of taurine ranged from 1.0 to 200 μg/mL, the concentration of taurine and peak area had a good correlation. The standard curve equation was Y=34182X+14599, r2=0.9999. The recoveries of the optimized method were 96.83%-100.17%. The limit of detection was 0.53 mg/100 g and the limit of quantitation was 1.76 mg/100 g. Conclusion The method is efficient and accurate, and is suitable for detecting the content of taurine in functional drinks, so as to provide theoretical support for daily supervision work.