2种微生物试剂盒法快速检测乳粉中叶酸含量

Rapid determination of the content of folic acid in milk power by two rapid microbiological kit methods

中文关键词:  叶酸  商业化试剂盒  微生物法  婴幼儿配方乳粉  快速检测

英文关键词:folic acid  commercial kit  microbiological method  infant formula milk power  rapid test

中文摘要:

      目的 对2种商业化试剂盒法检测婴幼儿配方乳粉中叶酸含量的结果进行对比研究。方法 通过制作标准曲线, 加标回收试验及样品重复性试验对2种试剂盒进行比对。结果 研究表明2种试剂盒的叶酸标准曲线相关系数均在0.999以上; 样品加标回收试验的加标回收率均在90%~100%之间, 符合试剂盒加标回收要求; 样品重复性试验结果相对标准偏差均小于5%。结论 2种商业化叶酸试剂盒具有操作简单、实验周期短、重复性较好等特点, 可快速检测婴幼儿配方乳粉中叶酸的含量。

英文摘要:

      Objective To compare the results of 2 commercial kits for determination of folic acid in infant formula milk powder. Methods The two standard kits were compared by making standard curves, the addition standard recovery tests and repeating the sample tests. Results The results showed that the folic acid standard curve correlation coefficients of the two kits were above 0.999. The recoveries of the samples were between 90% and 100%, which were in line with the standard recovery requirements of the kit. The relative standard deviations of the sample repeatability test results were less than 5%. Conclusion The 2 commercial folic acid kits have the characteristics of simple operation, short experimental period and good repeatability, and can quickly detect the content of folic acid in infant formula milk powder.