高效液相色谱法检测保健品中西地那非的含量的不确定度评定

Uncertainty evaluation for determination sildenafil in health products by high performance liquid chromatography

中文关键词:  高效液相色谱法  西地那非  保健品  不确定度

英文关键词:high performance liquid chromatography  sildenafil  health product  uncertainty

中文摘要:

      目的 评定高效液相色谱法(high performance liquid chromatography,HPLC)检测保健品中西地那非的含量的不确定度。方法 以保健品中西地那非非法添加为例, 采用高效液相色谱对样品进行定性筛查, 定量分析, 并对定量结果分析不确定度来源, 计算测量结果的相对扩展不确定度。结果 当保健品中西地那非含量为14.0 mg/g, 在95%的置信区间下, 其结果的扩展不确定度为0.36 mg (k=2)。结论 在影响检测结果的各分量中, 标准溶液配制过程引入的不确定度对结果影响较大。

英文摘要:

      Objective To evaluate the uncertainty of determination of sildenafil in health products by high performance liquid chromatography (HPLC). Methods Taking the illegal addition of sildenafil in health care products as an example, qualitative screening and quantitative analysis were performed by HPLC. The source of uncertainty was analyzed for quantitative results, and the relative extended uncertainty of the measurement results was calculated. Results When the content of sildenafil was 14.0 mg/g, the extended uncertainty of the results was 0.36 mg at the 95% confidence interval (k=2). Conclusion Among the components affecting the test results, the uncertainty introduced by the standard solution preparation process has a greater impact on the results.