改进的QuEChERS-超高效液相色谱-串联质谱法测定毛木耳中23种植物生长调节剂的残留量

Determination of 23 kinds of plant growth regulators residues in Auricularia cornea Ehrenb. by modified QuEChERS method and ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  PEP-2  QuEChERS  超高效液相色谱-串联质谱法  植物生长调节剂  毛木耳

英文关键词:pep-2  QuEChERS  ultra performance liquid chromatography-tandem mass spectrometry  plant growth regulator  auricularia cornea

中文摘要:

      目的 建立改进的QuEChERS-超高效液相色谱-串联质谱法测定毛木耳中23种植物生长调节剂的分析方法。方法 样品经1%乙酸乙腈溶液提取后, 比较了C18和PEP-2的净化和去除色素能力, 同时对提取试剂和PEP-2净化剂用量进行了优化, 采用超高效液相色谱-串联质谱测定。结果 在优化的条件下, 23种植物生长调节剂在相关范围内线性关系良好(r>0.999), 方法定量限为0.1~5 μg/kg, 在0.01、0.05、0.1 mg/kg 3个添加浓度下平行6次实验, 回收率为80.6%~116.6%, RSD为0.9%~11.2%。结论 该法满足国内外植物生长调节剂残留限量要求, 可为食用菌中植物生长调节剂残留检测提供参考。

英文摘要:

      Objective To establish a method for determination of 23 plant growth regulators in Auricularia cornea by modified QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry (UPLS-MS/MS). Methods The samples were extracted with 0.1% acetic acetonitrile solution, then the capacity of purification and pigment removal of C18 and PEP-2 were compared. At the same time, the amount of extraction reagent and PEP-2 purifier was optimized, and samples were detected by UPLC-MS/MS. Results Under the optimum conditions, 23 plant growth regulators had linear relationships (r>0.999), and the limits of quantitation were ranged from 0.1 to 5 μg/kg. The recoveries at 3 spiked levels (0.01, 0.05 and 0.1mg/kg) were 82.6%-116.6%, with the relative standard deviations (RSD) of 0.9%-11.2% (n=6). Conclusion This method is satisfied the demand of the plant growth regulators residue limits, which can provide technical support for detection of plant growth regulator residues in edible fungi.