高效液相色谱-三重四极杆-离子阱串联质谱法在食品分析中的应用

Application of high performance liquid chromatography-quadruple/linear ion trap mass spectrometry in food analysis

中文关键词:  高效液相色谱-三重四极杆-离子阱串联质谱法  食品  痕量分析  筛查  确证  基质效应  结构分析

英文关键词:quadruple /linear ion trap mass spectrometry, food, trace analysis, screening, identification, matrix effect, structure analysis

中文摘要:

      三重四极杆-离子阱串联质谱(quadruple/linear ion trap mass spectrometry, Q-Trap MS)不仅具有一般三重四极杆质谱的特征离子提取功能, 而且具有线性离子阱的离子捕获、富集等功能, 可以大大提高复杂基质中的检测选择性和灵敏度; Q-Trap MS技术独特的多反应监测-信息依赖性采集-增强子离子扫描模式可以同时获得多反应监测数据以及感兴趣的二级全扫描质谱图, 通过自建谱库比对可进行未知物的筛查确证及化合物结构分析。因此Q-Trap MS技术在食品科学领域发挥着越来越重要的作用。本文主要对高效液相色谱-三重四极杆-离子阱串联质谱法在食品痕量分析、高通量快速筛查、化合物确证、降低基质效应及化合物结构分析方面的应用进行了概述, 以期为Q-Trap MS技术在食品中的进一步发展应用提供参考。

英文摘要:

      Quadruple/linear ion trap mass spectrometry (Q-Trap MS) not only has the extraction function of the characteristic ion of general triple-quad mass, but also has the function of the ion capture and enrichment of linear ion trap. It can greatly improve the selectivity and sensitivity in complex matrix. The unique multiple- reaction monitoring (MRM)-information dependent acquisition-enhanced production of Q-Trap MS can get MRM data and interested second-order full-scan mass spectra at the same time. The self-established spectral database can be used for screening and confirmation of unknown substances and analysis of compound structure. Q-Trap MS technology is playing an increasingly important role in the field of food science. This paper summarized the applications of high performance liquid chromatography-quadruple/linear ion trap mass spectrometry (HPLC-Q-Trap-MS) in food trace analysis, high throughput rapid screening, compound identification and matrix effect reduction, compound structure analysis, so as to provide references for the further development and application of Q-Trap MS in food.