短波紫外线处理对3种链格孢毒素的降解作用研究

Study on degradation of 3 kinds of alternaria toxins by short-wave ultraviolet treatment

中文关键词:  链格孢毒素  毒素降解  短波紫外线辐照

英文关键词:alternaria toxin  toxin degradation  short-wave ultraviolet irradiation

中文摘要:

      目的 研究短波紫外线(UV-C)辐照对交链孢酚单甲醚(alternariol monomethyl ether, AME)和交链孢酚(alternariol, AOH)、腾毒素(tentoxin, TEN)3种链格孢毒素的降解效果。方法 测定不同辐照时间、不同辐照距离、不同pH值条件下、不同照射强度下UV-C辐照对3种毒素的影响, 采用高效液相色谱技术测定毒素含量。结果 经过UV-C辐照过后, 3种毒素的浓度均随着辐照时间的延长而降低, 随着辐照强度的增强而降低, 但是辐照距离与毒素溶液的pH值大小与毒素的降解情况并无显著关联。在pH为5, 毒素浓度为1.0 μg/mL, UV-C灯功率为36 W、辐照距离为25 cm条件下照射120 min后, AME、AOH、TEN 3种毒素的降解率分别为16.10%、63.92%、89.99%。结论 在偏酸性环境及长时间的UV-C辐照暴露下, UV-C对链格孢毒素有较为明显的降解作用。

英文摘要:

      Objective To investigate the degradation of 3 kinds of alternaria toxins including alternariol monomethyl ether (AME), alternariol (AOH) and tentoxin (TEN) by short-wave ultraviolet (UV-C) treatment. Methods The effects of UV-C irradiation on 3 kinds of alternaria toxins under different irradiation time, distances, pH values and irradiation intensities were studied, and the toxin content was determined by high performance liquid chromatography. Results After UV-C irradiation, the concentrations of the 3 kinds of toxins decreased with the extension of irradiation time, and the increase of the irradiation intensity, but the irradiation distance and the pH value of the toxin solution had no significant correlation with the degradation of the toxin. When pH was 5, the concentration of toxin was 1.0 μg/mL, the UV-C lamp power was 36 W, and the irradiation distance was 25 cm, the degradation rates of AME, AOH and TEN were 16.10%. 63.92%, 89.99%, respectively. Conclusion Under the acidic environment and long-term exposure to UV-C irradiation, UV-C has a significant degradation effect on alternaria toxin.