1株罕见的可疑沙门氏菌鉴定结果的确认与分析

Identification and analysis of the uncommonly suspected Salmonella

中文关键词:  沙门氏菌双相亚利桑那亚种  鉴定  盲样考核

英文关键词:Salmonella enterica subsp. diarizonae  identification  assessment of unknown sample

中文摘要:

      目的 确认盲样考核样品中分离到的罕见可疑沙门氏菌。方法 依据GB 4789.4-2016《食品安全国家标准 食品微生物检验 沙门氏菌检验》将可疑沙门氏菌通过分离纯化、生化鉴定、16S测序、Ribo-printer基因指纹鉴定、MALDI Biotyper质谱鉴定以及血清型分型等方法对其进行确认。结果 全自动细菌鉴定/药敏系统对该分离菌的鉴定结果为大肠埃希菌, 而16S测序结果为沙门氏菌属, 基因指纹鉴定结果为沙门氏亚利桑那亚种, 质谱鉴定结果为沙门氏菌双相亚利桑那亚种, 且血清型分型也表明该菌应为IIIb型, 综合判断该检出菌应为沙门氏菌双相亚利桑那亚种。结论 采用基于不同原理的多种鉴定手段联用的鉴定方法更能确保实验结果的可靠有效。

英文摘要:

      Objective To confirm of the uncommonly suspected Salmonella isolated from the unknown sample. Methods Isolation and purification of suspected Salmonella were conducted according to the GB 4789.4-2016 National food safety standard-Food microbiological examination-Salmonella, and it was confirmed by biochemical identification, 16S sequencing, Ribo-printer automatic microbial gene fingerprint identification system, MALDI Biotyper microbial identification time of flight mass spectrometry and serotyping. Results The identification result of automatic bacterial identification/drug sensitivity system was Escherichia coli. The identification result of 16S sequencing was Salmonella. The identification result of gene fingerprint identification system was Salmonella arizonae/IIIb. The identification result of was Salmonella enterica subsp. diarizonae, and serotyping also indicated that the type was IIIb, so the detected bacteria should be Salmonella enterica subsp. diarizonae. Conclusion The combination of multiple identification methods can ensure the reliability of the experimental results.