丁香酚在罗非鱼血浆中药代动力学研究

Study on pharmacokinetics of eugenol in tilapia plasma

中文关键词:  罗非鱼  丁香酚  血浆  药代动力学

英文关键词:tilapia  eugenol  plasma  pharmacokinetic

中文摘要:

      目的 在理想麻醉浓度条件下, 研究丁香酚在罗非鱼血浆中药代动力学。方法 选取体重为(250±10) g的罗非鱼放入丁香酚理想麻醉浓度溶液中药浴, 待完全麻醉后, 放入清水中复苏, 并在0~18 h间隔取样, 采用液相色谱串联质谱法检测血浆中丁香酚含量, DAS2.0软件计算血浆中丁香酚药代动力学参数。结果 药代动力学参数显示, 丁香酚在罗非鱼血浆中最高血药浓度Cmax为4.81 mg/L, 消除半衰期t1/2β为2.06 h, 平均滞留时间(mean residence time, MRT)为3.34 h, 药时曲线下面积(area under the curve, AUC)为14.55 mg/(L?h)。结论 丁香酚在罗非鱼血浆中半衰期短, 消除速率较快, 药代参数符合二室模型。

英文摘要:

      Objective To investigate the pharmacokinetics of eugenol in tilapia plasma under the optimal level of anesthesia. Methods The tilapia with body weight of (250±10) g were bathed in eugenol solution for anesthesia, after complete anesthesia, the tilapia were put into water to recovery. The plasma of tilapia collected at 0~18 h was detected by liquid chromatography-tandem mass spectrometry. The pharmacokinetic parameters of eugenol in plasma were calculated by DAS2.0 software. Results The results showed that the peak concentrations (Cmax) in tilapia plasma was 4.81 mg/L and the elimination half-life (t1/2β) was 2.06 h. In addition, the mean residence time (MRT0-∞) was 3.34 h and the area under the concentration-time curve (AUC0-∞) was 14.55 mg/(L?h). Conclusion Eugenol has a short half-life and its elimination is relatively fast in tilapia plasma, which is the pharmacokinetic parameters fitted to two-compartment model.