Oasis PRiME HLB-高效液相色谱-串联质谱法测定黄鱼中的碱性嫩黄O

Determination of auramine O in yellow croaker by Oasis PRiME HLB-high performance liquid chromatography-tandem mass spectrometry

中文关键词:  Oasis PRiME HLB  高效液相色谱-串联质谱法  黄鱼  碱性嫩黄O

英文关键词:Oasis PRiME HLB  high performance liquid chromatography-tandem mass spectrometry  yellow croaker  auramine O

中文摘要:

      目的 建立Oasis PRiME HLB-高效液相色谱-串联质谱法测定黄鱼中的碱性嫩黄O的分析方法。方法 样品经乙醇提取液充分提取, 经涡旋、超声、低温高速离心后, 上清液过Oasis PRiME HLB固相萃取柱净化, 流出液经氮吹吹干, 复溶, 待质谱分析。结果 在1.5、15.0、30 μg/L 3个加标浓度下, 回收率为85.3%~ 94.8%, 相对标准偏差为3.0%~7.2%, 方法定量限为1 μg/kg。结论 该方法前处理十分简便, 固相萃取净化水平高, 色谱串联质谱分析快速灵敏, 回收率高, 灵敏度高, 适合黄鱼中非食用物质碱性嫩黄O的定性定量 分析。

英文摘要:

      Objective To establish a method for determination of auramine O in yellow croaker by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods The samples were fully extracted by ethanol extract, and purified by Oasis PRiME HLB solid phase extraction column after centrifugation at low temperature and high speed. The effluent was dried by nitrogen blowing and resolved, and then analyzed by mass spectrometry. Results At the spiked concentrations of 1.5, 15.0, and 30 μg/L, the recoveries ranged from 85.3%?94.8%. The relative standard deviation was 3.0%?7.2%, and the limit of quantitative of the method was 1 μg/kg. Conclusion This method is simple, rapid, sensitive, and sensitive, which is suitable for the qualitative and quantitative analysis of non-edible alkaline auramine O in yellow croaker.