固相萃取-高效液相色谱法测定食品基质中5种不同合成红色着色剂

Determination of 5 kinds of different synthetic red colorants in food substrates by solid phase extraction-high performance liquid chromatography

中文关键词:  固相萃取  高效液相色谱法  食品  红色合成着色剂

英文关键词:solid phase extraction  high performance liquid chromatography  food  synthetic red colorant

中文摘要:

      目的 建立一种固相萃取-高效液相色谱法(solid phase extraction-high performance liquid chromatography, SPE-HPLC)测定食品中5种人工合成红色着色剂(新红、苋菜红、胭脂红、诱惑红、赤藓红)的方法。方法 比较ProElut PWA-2, Cleanert PWAX和Sep-Pak Plus QMA 3种不同SPE小柱的净化效果, 比较Eclipse XDB C18、Venusil XBP C18及Agilent TC-C18 3种色谱柱对5种着色剂的分离情况, 按照GB 5009.35-2016《食品中合成着色剂的测定》对食品中5种着色剂进行测定。结果 ProElut PWA-2 SPE固相萃取小柱进行样品净化效果最优, Agilent TC-C18色谱柱在10 min内有较好的分离度。加标浓度为0.5~50 mg/kg时, 该方法回收率为90.3%~103.9%, 相对标准偏差(relative standard deviation, RSD)值为1.18%~7.11%。结论 该方法快速、准确, 适合用于不同食品基质中5种合成着色剂定性、定量测定。

英文摘要:

      Objective To establish a method for determination of 5 kinds of synthetic red colorants (new red, amaranth, carmine, temptation red and erythromycin) in food substrates by solid phase extraction-high performance liquid chromatography. Methods The purification effects of three different SPE cartridges (ProElut PWA-2, Cleanert PWAX and Sep-Pak Plus QMA) were compared, and the separation of 5 colorants by Eclipse XDB C18, Venusil XBP C18 and Agilent TC-C18 columns were compared. Five kinds of colorants in food were detected according to GB 5009.35-2016 Determination of synthetic colorants in food. Results The ProElut PWA-2 SPE solid phase extraction cartridge provided the best sample purification. The Agilent TC-C18 column had good resolution in 10 min. When the concentrations were 0.5-50 mg/kg, the recovery rates of the method were 90.3%-103.9%, and the relative standard deviations (RSDs) were 1.18%-7.11%. Conclusion This method is rapid and accurate, which is suitable for qualitative and quantitative determination of 5 kinds of synthetic colorants in different food matrices.