胶束电动毛细管色谱法分析3种交链孢毒素主成分含量

Analysis of the main component of 3 kinds of alternaria toxins by micellar electrokinetic chromatographic

中文关键词:  胶束电动毛细管色谱法  交链孢毒素  交链孢酚  交链孢酚单甲醚  交链孢菌酮酸

英文关键词:micellar electrokinetic chromatography  alternaria toxin  alternariol  alternariol monomethyl ether  tenuazonic acid

中文摘要:

      目的 建立胶束电动毛细管色谱法(micellar electrokinetic chromatographic, MEKC)测定3种交链孢毒素[交链孢酚(alternariol, AOH)、交链孢酚单甲醚(alternariol monomethyl ether, AME)和交链孢菌酮酸(tenuazonic acid, TeA)]主成分含量的新分析方法。方法 以未涂层熔融石英毛细管柱(50 μm×30 cm, 20 cm, i.d.)为分离柱, 分别以3种分离缓冲溶液[40 mmol/L Na2B4O7-120 mmol/L 十二烷基硫酸钠(sodium dodecyl sulfate, SDS)(含体积分数为5%的乙腈)、30 mmol/L Na2B4O7-40 mmol/L SDS(含体积分数为30%的异丙醇)和60 mmol/L Na2B4O7-180 mmol/L脱氧胆酸钠]及2种样品缓冲溶液[8 mmol/L硼砂(含体积分数为57%的甲醇用于AOH和TeA)、8 mmol/L硼砂(含体积分数为57%的异丙醇用于AME)]分析AOH、AME和TeA的主成分含量。结果 AOH、AME、TeA的方法精密度分别为0.05%、0.01%和0.21%(n=5), 检出限分别为5、15和0.5 mg/L。主成分含量分别为99.94%、99.78%和97.28%, 且与高效液相色谱法(high performance liquid chromatography, HPLC)的结果无显著差异(α=0.05)。结论 MEKC法可作为HPLC法测定3种交链孢毒素主成分含量方法的互补方法, 能够满足3种交链孢菌毒素主成分含量分析的要求。

英文摘要:

      Objective To establish a new method for the main component analysis of alternariol (AOH), alternariol monomethyl ether (AME) and tenuazonic acid (TeA) by micellar electrokinetic chromatographic (MEKC). Methods An uncoated fused-silica capillary (50 μm×30 cm, 20 cm) was used for separation. Three kinds of separation buffer [40 mmol/L Na2B4O7-120 mmol/L SDS containing 5% (V/V) acetonitrile, 30 mmol/L Na2B4O7- 40 mmol/L SDS containing 30% (V/V) isopropanol and 60 mmol/L Na2B4O7-180 mmol/L sodium doexycholate] combining with 2 kinds of sample buffer [8 mmol/L Na2B4O7 containing 57% (V/V) methanol for AOH and TeA, 8 mmol/L Na2B4O7 containing 57% (V/V) isopropanol for AME] were used for main component analysis of 3 kinds of alternaria toxins. Results The precision of the method of AOH, AME and TeA was 0.05%, 0.01% and 0.21% (n=5), respectively. The limit of detection was 5, 15 and 0.5 mg/L, respectively. The main components of the 3 kinds of alternaria toxins were 99.94%、99.78% and 97.28%, respectively. There was no significant difference between the 2 methods of MEKC and high performance liquid chromatography (HPLC) (α=0.05). Conclusion The established MEKC is an alternative method to the HPLC, which can meet the requirements for the main component analysis of the above 3 kinds of toxins.