发芽处理后花生中白藜芦醇积累情况的高效液相色谱法的研究

Study on the accumulation of resveratrol in peanut after germination by high performance liquid chromatography

中文关键词:  白藜芦醇  发芽花生  高效液相色谱  胁迫剂

英文关键词:Resveratrol  Germinated peanut  High performance liquid chromatography  Stress agent

中文摘要:

      花生中白藜芦醇是一种天然存在的生物活性很强的多酚类物质。目的：本实验通过高效液相色谱法研究不同的胁迫试剂提高花生发芽过程中白藜芦醇的积累。方法：通过三种不同的花生发芽培养液(无菌去离子水，600 μM CuSO4溶液和10 mg/L壳聚糖溶液)对花生发芽过程进行处理，并且建立了提取和净化花生中白藜芦醇的前处理方法。结果：不同胁迫试剂处理发芽花生对白藜芦醇的富集影响不同。用无菌去离子水对发芽花生处理中72 h白藜芦醇含量最高为130.2 ng/g，用600 μM CuSO4溶液对发芽花生处理中24 h白藜芦醇含量最高为568.7 ng/g，用10 mg/ L的壳聚糖溶液处理五天后白藜芦醇含量最高为383.6 ng/g。结论：在三种处理方式中，根据发芽产品的需要，用CuSO4和壳聚糖都可以作为胁迫试剂对发芽花生进行处理以此来提高花生不同发芽阶段白藜芦醇的含量。

英文摘要:

      Resveratrol in peanuts is a naturally occurring, highly bioactive polyphenolic substance.In this experiment, different stress agents were studied to improve the accumulation of resveratrol during peanut germinationby high performance liquid chromatography. The peanut germination process was treated by three different peanut germination mediums (sterile deionized water, 600 μM CuSO₄ solution and 10 mg/L chitosan solution), and the extraction and purification of resveratrol in peanuts was established.The content of resveratrol was determined by liquid chromatography. The results showed that different stress agents had different effects to enrich resveratrol in germinated peanuts. The highest content of resveratrol in the treatment of germinated peanut was 130.2 ng/g with sterile deionized water, and the highest content of resveratrol was 568.7 ng/g in the treatment of germinated peanut with 600 μM CuSO4 solution. After treatment with 10 mg/L chitosan solution for five days, the resveratrol content was up to 383.6 ng/g.