高效液相色谱-串联质谱法测定功能饮料中肌醇的含量

Determination of inositol in functional drink by high performance liquid chromatography with tandem mass spectrometry

中文关键词:  肌醇  功能饮料  高效液相色谱-串联质谱

英文关键词:inositol  functional drink  high performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立液相色谱-串联质谱法测定功能饮料中肌醇含量。方法 功能饮料振荡混匀后取样0.2 mL，用50%的乙腈水稀释500倍，过0.22 μm的微孔滤膜后直接进样，经BEH Amide色谱柱分离，用串联四极杆质谱采用多反应监测模式进行测定，外标曲线法定量。结果 肌醇在0.05～2.0 μg/mL范围内线性关系良好，线性相关系数r为0.9999，检出限和定量限分别为0.65 mg/L和2.15 mg/L；加标浓度为0.025、0.125和0.25 mg/mL的平均回收率分别为103.5%、100.0%和101.8%，批内精密度分别为3.3%、1.1%和1.5%，批间精密度分别为2.4%、1.9%和2.1%。结论 该方法前处理简单、快速、准确、灵敏，可以满足实际检测分析的需要。

英文摘要:

      Objective To establish a method for the determination of inositol in functional drink by high performance liquid chromatography-tandem mass spectrometry. Methods After shaking and mixing, the functional drink was diluted 500 times with 50% acetonitrile water, and directly injected after passing through 0.22 μm microporous membrane filter. Inositol was separated by BEH Amide column, determined by tandem quadrupole mass spectrometry using multi-reaction monitoring mode, and quantified by external standard method. Results The inositol had a good linear relationship in the concentration range of 0.05-2.0 μg/mL with correlation coefficients of 0.9999. The detection limit and quantitative limit were 0.65 mg/L and 2.15 mg/L, respectively. The average recovery of inositol at 0.025, 0.125 and 0.25 mg/mL were 103.5%, 100.0% and 101.8%, respectively, and intra-batch precision were 3.3%, 1.1% and 1.5%, respectively，inter-batch precision were 2.4%, 1.9% and 2.1%, respectively. Conclusion The method is simple, accurate and sensitive which can meet the requirements of practical analysis.