TaqMan荧光PCR法快速检测太平洋鳕鱼源性成分

Rapid identification of Gadus macrocephalus source componentsby TaqMan fluorescent PCR

中文关键词:  CoxⅠ  荧光PCR  太平洋鳕鱼

英文关键词:CoxⅠ  TaqMan real-time fluorescence PCR  Gadus macrocephalus

中文摘要:

      目的 建立TaqMan荧光PCR法检测太平洋鳕鱼源性成分的方法。方法 以太平洋鳕鱼线粒体细胞色素氧化酶CoxⅠ检测靶标, 设计特异性引物和探针, 进行特异性和灵敏度试验, 并对市售样品进行检测。结果 该方法引物探针特异性和灵敏度较好, 可以良好区分近属鳕鱼和其他科鱼类, 灵敏度达到0.04 ng/μL。市售样品中, 90.9%太平洋鳕鱼切片及71.4%太平洋鳕鱼罐头中检出太平洋鳕鱼成分。结论 该方法的特异性和灵敏度较好, 适合用于大多数制品中太平洋鳕鱼成分的真实性甄别。

英文摘要:

      Objective To establish a method of rapid identification of the authenticity of Gadus macrocephalussource components by TaqMan fluorescent PCR. Methods Targets were detected by Gadus macrocephalus mitochondrial cytochrome oxidase CoxI, specific primers and probes were designed, specificity and sensitivity tests were performed, and commercially available samples were tested. Result The primers of this method had good specificity and sensitivity, and could well distinguish between Gadus macrocephalus and other species of fish, with a sensitivity of 0.04 ng/μL.Among the commercially available samples, Gadus macrocephalus were found in 90.9% of Gadus macrocephalus slices and 71.4% of Gadus macrocephalus canned food. Conclusion The method has good specificity and sensitivity, which is suitable for the authenticity screening of Gadus macrocephalus componentsin most products.