分子生物学鉴定印度进口花生中的花生豆象的探究

Preliminary exploration of identifying Caryedon serratus (Olivier) in peanuts from India by molecular biology techniques and systems

中文关键词:  花生豆象  生命周期  细胞色素C氧化酶亚基I  PCR  系统进化

英文关键词:Caryedon serratus  life cycle  cytochrome C oxidase I  PCR  phylogeny

中文摘要:

      目的 探究以分子生物学技术和系统进化学手段鉴定具有完整生命周期的花生豆象(印度)的方法。方法 对花生豆象线粒体细胞色素C氧化酶亚基I (COI)基因进行PCR扩增, 获得COI基因序列, 再利用最大简约法(maximum parsimony, MP)构建花生豆象与其他14种豆象基于COI基因片段序列的分子系统进化树, 分析亲缘进化关系。结果 通过GenBank BLAST证明所得序列为花生豆象的COI基因序列, 由GenBank获得登录号。根据系统进化树得出同一地区的四纹豆象和花生豆象亲缘关系较近。结论 本研究为采用分子生物学检疫鉴定进境花生豆象奠定了基础。

英文摘要:

      Objective To explore the methods for the identification of Caryedon serratus (Olivier) in peanuts with a complete life cycle from India by molecular biology techniques and systems. Methods The mitochondria cytochrome Coxidase I (COI) gene of Caryedon serratus mitochondria was amplified by PCR, and the COI gene sequence was obtained. Then the phylogenetic tree of Caryedon serratus and 14 kinds of other bean weevils based on COI gene fragment sequence was constructed by maximum parsimony (MP) method, and the phylogenetic relationship was analyzed. Results The sequence was confirmed as COI gene of Caryedon serratus by GenBank BLAST, and submitted to GenBank for a login number. The phylogenetic tree showed that Callosobruchus maculates (Fabricius) and Caryedon serratus in the same area had a close phylogenetic relationship. Conclusion This study lays a foundation for the use of molecular biology techniques and systems to identify Caryedon serratus (Olivier) in imported peanuts.